| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,460,048 | C→T | A332V (GCG→GTG) | paaH → | 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,460,048 | 0 | C | T | 100.0% | 113.9 / NA | 33 | A332V (GCG→GTG) | paaH | 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/19); total (14/19) | |||||||||||
TTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGG > NC_000913/1459928‑1460180 | ttgttgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGc > 2:405624/1‑139 (MQ=255) tgGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGc < 1:405624/139‑1 (MQ=255) ggCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc < 2:766619/139‑1 (MQ=255) ggCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc < 2:665703/139‑1 (MQ=255) aGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTa < 2:34867/139‑1 (MQ=255) ttAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGc < 2:145671/139‑1 (MQ=255) gCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCggg < 2:656648/139‑1 (MQ=255) ccAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCa < 2:211262/139‑1 (MQ=255) aTGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAgg > 1:4611/1‑139 (MQ=255) gaaaagaaaaGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCa < 1:453910/127‑1 (MQ=255) gaaaagaaaaGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGAAACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCa > 2:453910/1‑127 (MQ=255) gtgtCACGGAAATTGACG‑‑GTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGa > 2:192066/1‑139 (MQ=255) gtCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc > 2:259355/1‑79 (MQ=255) gtCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc < 1:259355/79‑1 (MQ=255) aTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCt < 1:730978/50‑1 (MQ=255) aTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCt > 2:730978/1‑50 (MQ=255) aTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc > 2:115832/1‑70 (MQ=255) aTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc < 1:701140/70‑1 (MQ=255) aTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc < 1:115832/70‑1 (MQ=255) aTTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGcc > 2:701140/1‑70 (MQ=255) ttATTGATTGAGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCa < 2:55250/139‑1 (MQ=255) ttgattgaGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAgg < 2:496367/139‑1 (MQ=255) attgaGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGAcc < 2:245970/93‑1 (MQ=255) attgaGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGAcc > 1:245970/1‑93 (MQ=255) tgaGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCg > 1:137236/1‑52 (MQ=255) tgaGACACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCg < 2:137236/52‑1 (MQ=255) agaCACAAGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATtta > 1:665582/1‑139 (MQ=255) aaGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCAt < 2:594622/85‑1 (MQ=255) aaGGCGAGACGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCAt > 1:594622/1‑85 (MQ=255) gaCGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGc < 2:588852/120‑1 (MQ=255) gaCGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGc > 1:588852/1‑120 (MQ=255) gaCGGCACAGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAAcag < 2:665582/139‑1 (MQ=255) cacaGGTGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAggg > 1:476814/1‑139 (MQ=255) | TTGTTGGCCTGGAAGCGGTAAGCGACAGTTTTAGCCCAATGAAAGTAGAAAAGAAAAGTGACGGTGTCACGGAAATTGACGATGTTTTATTGATTGAGACACAAGGCGAGACGGCACAGGCGCTGGCAATACGACTGGCACGCCCGGTGGTAGTGATCGATAAAATGGCGGGCAAGGTGGTGACCATTGCTGCTGCAGCGGTGAACCCGGACTCAGCGACCCGCAAGGCCATTTATTACCTGCAACAGCAGGG > NC_000913/1459928‑1460180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |