| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,629,649 | A→G | L422P (CTG→CCG) | ydfI ← | putative NAD‑dependent D‑mannonate oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,629,649 | 0 | A | G | 100.0% | 86.7 / NA | 25 | L422P (CTG→CCG) | ydfI | putative NAD‑dependent D‑mannonate oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/10); total (15/10) | |||||||||||
GTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACAGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCAACATCC > NC_000913/1629520‑1629784 | gTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCAc > 2:605103/1‑139 (MQ=255) acTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTccc < 1:605103/139‑1 (MQ=255) tGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTtc > 1:259041/1‑139 (MQ=255) tGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTtc < 1:623692/139‑1 (MQ=255) gCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAac < 1:234308/139‑1 (MQ=255) tcaCCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTTCCTGTTCATCAACACCACCGa < 1:619040/139‑1 (MQ=255) gACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAgcgc > 2:780091/1‑135 (MQ=255) gACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAgcgc < 1:780091/135‑1 (MQ=255) tGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTa < 2:489989/46‑1 (MQ=255) tGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTa > 1:489989/1‑46 (MQ=255) ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTccc > 2:266283/1‑50 (MQ=255) ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTccc < 1:266283/50‑1 (MQ=255) ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCg > 1:468912/1‑139 (MQ=255) ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCg > 2:386713/1‑139 (MQ=255) tCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAAc > 2:738145/1‑139 (MQ=255) tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGccc < 2:142394/90‑1 (MQ=255) tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGccc > 1:142394/1‑90 (MQ=255) tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAg > 1:292611/1‑139 (MQ=255) tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAg > 1:401967/1‑139 (MQ=255) cAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATACAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCaa > 2:630976/1‑139 (MQ=255) gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCaa > 1:299599/1‑45 (MQ=255) gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCaa < 2:299599/45‑1 (MQ=255) gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATgcg < 2:450895/111‑1 (MQ=255) gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATgcg > 1:450895/1‑111 (MQ=255) aaCGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCAACATcc > 2:721834/1‑139 (MQ=255) | GTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACAGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCAACATCC > NC_000913/1629520‑1629784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |