Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,667,344	T→C	*407* (TAA→TAG)	mlc ←	glucosamine anaerobic growth regulon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,667,344	0	T	C	100.0%	131.6 / NA	36	*407* (TAA→TAG)	mlc	glucosamine anaerobic growth regulon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/18);  total (18/18)

GCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGC  >  NC_000913/1667216‑1667478                                                                                                                                 |                                                                                                                                       gCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCa                                                                                                                              <  2:371663/139‑1 (MQ=255)    tCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACa                                                                                                                           >  1:13645/1‑139 (MQ=255)                   ttATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAg                                                                                                            <  2:468645/139‑1 (MQ=255)                     aTGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGaa                                                                                                          <  2:13645/139‑1 (MQ=255)                           cacaAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGaa                                                                                                          >  2:58132/1‑133 (MQ=255)                           cacaAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGaa                                                                                                          <  1:58132/133‑1 (MQ=255)                                  ataAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATcg                                                                                             >  2:368379/1‑139 (MQ=255)                                     aaCCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGa                                                                                                                         <  1:130021/108‑1 (MQ=255)                                     aaCCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGa                                                                                                                         >  2:130021/1‑108 (MQ=255)                                           gAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTa                                                                                    >  2:133415/1‑139 (MQ=255)                                                      aCCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAgcg                                                                         >  1:693015/1‑139 (MQ=255)                                                         ttGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAg                                                                                                            >  2:734926/1‑101 (MQ=255)                                                         ttGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAg                                                                                                            <  1:734926/101‑1 (MQ=255)                                                                    aGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGcc                                                           <  1:598743/139‑1 (MQ=255)                                                                          gCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGtt                                                     >  1:554490/1‑139 (MQ=255)                                                                          gCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGtt                                                     >  2:186545/1‑139 (MQ=255)                                                                                   gCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTg                                            >  2:383829/1‑139 (MQ=255)                                                                                       aaaTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTa                                        <  1:133415/139‑1 (MQ=255)                                                                                        aaTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGt                                         <  1:283577/137‑1 (MQ=255)                                                                                        aaTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGt                                         >  2:283577/1‑137 (MQ=255)                                                                                                   cgcAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTg                            >  1:696038/1‑139 (MQ=255)                                                                                                   cgcAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTg                            >  1:711510/1‑139 (MQ=255)                                                                                                     cAAATTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGAt                          <  1:383829/139‑1 (MQ=255)                                                                                                       aaTTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGt                                         >  2:290050/1‑122 (MQ=255)                                                                                                       aaTTTTGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGt                                         <  1:290050/122‑1 (MQ=255)                                                                                                         ttttGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGc                      <  2:497517/139‑1 (MQ=255)                                                                                                         ttttGGTAGAACAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGc                      <  2:696038/139‑1 (MQ=255)                                                                                                                  aaCAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGAt                          <  2:293066/126‑1 (MQ=255)                                                                                                                  aaCAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGAt                          >  1:293066/1‑126 (MQ=255)                                                                                                                   aCAGTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACg            <  2:693015/139‑1 (MQ=255)                                                                                                                      gTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATcg                                                                                             <  2:307205/55‑1 (MQ=255)                                                                                                                      gTTAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATcg                                                                                             >  1:307205/1‑55 (MQ=255)                                                                                                                       ttAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTa                                        <  2:572784/107‑1 (MQ=255)                                                                                                                       ttAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTa                                        >  1:572784/1‑107 (MQ=255)                                                                                                                       ttAAAAAATGCTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAgg        >  1:423707/1‑139 (MQ=255)                                                                                                                             aaTGCTAGCCCGGCACCAGACGAATCAGCAGCGAACCGTTATACATCGCGTCTTTTACCAGGGCAGCGCCTGCCATCGAGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACGATACGCAGGAAGGGc  <  2:233525/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       GCATCACTAACTCCACCGTTATGCTTCACAAATATAAACCAGGAAAATAATTAACCTTGAAAGTCTAAGTTATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGTTAGAAAACTGAGTACTCTCAACGCTGATGTGCTGACTATACGCAGGAAGGGC  >  NC_000913/1667216‑1667478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑