Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,541	A→T	I58F (ATC→TTC)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,541	0	A	T	100.0%	128.2 / NA	38	I58F (ATC→TTC)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/20);  total (18/20)

CAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAG  >  NC_000913/1806430‑1806675                                                                                                                |                                                                                                                                       cAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCg                                                                                                             >  1:583387/1‑139 (MQ=255)              aaGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTc                                                                                                >  2:347610/1‑139 (MQ=255)               aGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               <  2:125240/139‑1 (MQ=255)                                   cACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               >  1:228654/1‑119 (MQ=255)                                   cACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               <  2:228654/119‑1 (MQ=255)                                   cACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGt                                                                           >  2:505735/1‑139 (MQ=255)                                      cGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTccc                                                                        >  1:580451/1‑139 (MQ=255)                                        gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  2:411966/1‑139 (MQ=255)                                        gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  2:327458/1‑139 (MQ=255)                                        gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  1:506080/1‑139 (MQ=255)                                        gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  1:64502/1‑139 (MQ=255)                                                   cGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGaa                                                                                                           <  2:703562/91‑1 (MQ=255)                                                   cGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGaa                                                                                                           >  1:703562/1‑91 (MQ=255)                                                    gggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAg                                                          <  2:5547/139‑1 (MQ=255)                                                     ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCg                                                                                                             >  2:601109/1‑87 (MQ=255)                                                     ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCg                                                                                                             <  1:601109/87‑1 (MQ=255)                                                     ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     <  1:134406/127‑1 (MQ=255)                                                     ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     >  2:134406/1‑127 (MQ=255)                                                     ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGa                                                         >  2:718820/1‑139 (MQ=255)                                                          ggcggcATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCa                                                    <  2:506080/139‑1 (MQ=255)                                                          ggcggcATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCa                                                    >  2:379191/1‑139 (MQ=255)                                                                    aCGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGAc                                          <  2:583387/139‑1 (MQ=255)                                                                        cGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCgg                                      <  1:718820/139‑1 (MQ=255)                                                                          cccGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCa                                    <  1:411966/139‑1 (MQ=255)                                                                               gcCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               >  1:689479/1‑75 (MQ=255)                                                                               gcCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               <  2:689479/75‑1 (MQ=255)                                                                                ccATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAAttt                              <  1:235024/139‑1 (MQ=255)                                                                                   ttGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     >  2:395769/1‑97 (MQ=255)                                                                                   ttGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     <  1:395769/97‑1 (MQ=255)                                                                                   ttGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTaca                           <  1:347610/139‑1 (MQ=255)                                                                                      cccATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGt                        <  2:51545/139‑1 (MQ=255)                                                                                             tGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACAtgtg                 <  1:505735/139‑1 (MQ=255)                                                                                                     gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGcc                                                     >  2:61682/1‑95 (MQ=255)                                                                                                     gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGcc                                                     <  1:61682/95‑1 (MQ=255)                                                                                                     gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGaagc            <  1:268277/136‑1 (MQ=255)                                                                                                     gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGaagc            >  2:268277/1‑136 (MQ=255)                                                                                                            aGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGTAGAATTTACACGTACATGTGGAAGCAAGCGGTGAg  <  1:327458/139‑1 (MQ=255)                                                                                                            aGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAg  <  2:580451/139‑1 (MQ=255)                                                                                                                |                                                                                                                                       CAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAG  >  NC_000913/1806430‑1806675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑