Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,173	T→C	L414L (TTA→CTA)	ydjN →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,173	0	T	C	100.0%	88.9 / NA	25	L414L (TTA→CTA)	ydjN	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

GCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGA  >  NC_000913/1812050‑1812300                                                                                                                            |                                                                                                                                gCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAAc                                                                                                                  <  1:344162/139‑1 (MQ=255)       cTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTGGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTa                                                                                                            >  2:554594/1‑139 (MQ=255)                      aCCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTa                                                                                             >  2:589598/1‑139 (MQ=255)                         gTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACgg                                                                                          >  2:93683/1‑139 (MQ=255)                         gTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACgg                                                                                          >  2:309337/1‑139 (MQ=255)                                           gCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCt                                                                        >  1:709639/1‑139 (MQ=255)                                                                 cTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGAc                                                  <  1:93683/139‑1 (MQ=255)                                                                     cgccgcACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGAccagc                                              >  1:416079/1‑139 (MQ=255)                                                                                     ctgcctgcGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa                              >  1:534108/1‑139 (MQ=255)                                                                                       gcctgcGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTa                                                                              <  2:79425/89‑1 (MQ=255)                                                                                       gcctgcGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTa                                                                              >  1:79425/1‑89 (MQ=255)                                                                                        cctgcGATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCg                           >  2:282787/1‑139 (MQ=255)                                                                                             gATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGc                                       >  1:54934/1‑122 (MQ=255)                                                                                             gATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGc                                       <  2:54934/122‑1 (MQ=255)                                                                                             gATGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGACaaa                      >  1:410230/1‑139 (MQ=255)                                                                                              aTGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAg                     <  2:534108/139‑1 (MQ=255)                                                                                              aTGGGCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAg                     <  1:554594/139‑1 (MQ=255)                                                                                                  gCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGtt                                                                                      >  1:562369/1‑70 (MQ=255)                                                                                                  gCCTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGtt                                                                                      <  2:562369/70‑1 (MQ=255)                                                                                                   ccTGCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCAtt                <  2:113239/139‑1 (MQ=255)                                                                                                      gCCAGTAACCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTg             >  2:771580/1‑139 (MQ=255)                                                                                                             aCCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCAtt                <  1:468750/129‑1 (MQ=255)                                                                                                             aCCCTGGTGGCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCAtt                >  2:468750/1‑129 (MQ=255)                                                                                                                 tggtggCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAga  <  1:282787/139‑1 (MQ=255)                                                                                                                 tggtggCGCTGCTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAga  <  2:410230/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                GCGACGCTGGTCGGTATTGTTACCGTTAGTTCCGCAGGCGTTGCCGGTGTCGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGA  >  NC_000913/1812050‑1812300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑