Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,836,204	C→T	T44T (ACC→ACT)	ynjB →	putative ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,836,204	0	C	T	100.0%	57.4 / NA	18	T44T (ACC→ACT)	ynjB	putative ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

GATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGC  >  NC_000913/1836072‑1836329                                                                                                                                     |                                                                                                                              gatgCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAAtt                                                                                                                         <  2:727953/139‑1 (MQ=255)       ccATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGc                                                                                                                   >  2:471864/1‑139 (MQ=255)                    ttGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTAtc                                                                                                               <  1:323228/130‑1 (MQ=255)                    ttGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTAtc                                                                                                               >  2:323228/1‑130 (MQ=255)                      gCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTg                                                                                                    >  1:404528/1‑139 (MQ=255)                         gggATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAgc                                                                                                 >  1:348127/1‑139 (MQ=255)                                          tttCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCa                                                                                <  1:471864/139‑1 (MQ=255)                                                       acaTGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAAc                                                                   <  2:348127/139‑1 (MQ=255)                                                                            aaTTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGaaa                                                                                     <  1:186329/100‑1 (MQ=255)                                                                            aaTTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGaaa                                                                                     >  2:186329/1‑100 (MQ=255)                                                                                     tGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTa                                                                             <  2:371177/99‑1 (MQ=255)                                                                                     tGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTa                                                                             >  1:371177/1‑99 (MQ=255)                                                                                                 gCAAACCGTCTGGTTTAACGCCTGGGGCGGCTATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAg                         <  2:404528/139‑1 (MQ=255)                                                                                                  cAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAgc                        <  1:412271/139‑1 (MQ=255)                                                                                                          cTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGt                                                                                                     >  2:144423/1‑54 (MQ=255)                                                                                                          cTGGTTTAACGCCTGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGt                                                                                                     <  1:144423/54‑1 (MQ=255)                                                                                                                       tGGGGCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAg   >  2:518305/1‑139 (MQ=255)                                                                                                                        ggggCGGCGATACTGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGc  <  1:504841/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                              GATGCGCCATTGTGGGTGGTTGCTGGGATTGTTATCGCTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGC  >  NC_000913/1836072‑1836329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑