Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,189,791	A→G	Y374H (TAT→CAT)	yehB ←	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,189,791	0	A	G	100.0%	93.8 / NA	27	Y374H (TAT→CAT)	yehB	putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

GCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT  >  NC_000913/2189656‑2189917                                                                                                                                        |                                                                                                                               gCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAg                                                                                                                             <  2:223847/139‑1 (MQ=255)                cGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAg                                                                                                                             <  2:731099/124‑1 (MQ=255)                cGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAg                                                                                                                             >  1:731099/1‑124 (MQ=255)                  ccGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa                                                                                                            >  2:42919/1‑139 (MQ=255)                                   aTGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATaa                                                                                                                    <  2:692860/114‑1 (MQ=255)                                   aTGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATaa                                                                                                                    >  1:692860/1‑114 (MQ=255)                                   aTGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAAccc                                                                                           >  2:243117/1‑139 (MQ=255)                                      cGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGcc                                                                                        <  1:42919/139‑1 (MQ=255)                                            aGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACa                                                                                  >  2:348645/1‑139 (MQ=255)                                                   tCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACa                                                                                  <  2:22765/132‑1 (MQ=255)                                                   tCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACa                                                                                  >  1:22765/1‑132 (MQ=255)                                                      aCGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACttt                                                                        <  1:243117/139‑1 (MQ=255)                                                            aTGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGc                                                                  >  1:155722/1‑139 (MQ=255)                                                                    aaTGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCt                                                          >  1:659644/1‑139 (MQ=255)                                                                         gtgtATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAat                                                     <  2:706291/139‑1 (MQ=255)                                                                              ttCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGc                                                >  1:255074/1‑139 (MQ=255)                                                                                     cagccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGAcc                                         <  2:155722/139‑1 (MQ=255)                                                                                      agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa                                                                                                            <  2:632039/71‑1 (MQ=255)                                                                                      agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa                                                                                                            <  2:178253/71‑1 (MQ=255)                                                                                      agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa                                                                                                            >  1:632039/1‑71 (MQ=255)                                                                                      agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa                                                                                                            >  1:178253/1‑71 (MQ=255)                                                                                        cccccAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGGCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCCGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTAAGTCccgc                                      >  2:590511/1‑139 (MQ=255)                                                                                        cccccAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACccgc                                      >  2:581681/1‑139 (MQ=255)                                                                                                         taataaTTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCg                     >  2:481661/1‑139 (MQ=255)                                                                                                                            tCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGc                                                              >  2:25975/1‑79 (MQ=255)                                                                                                                            tCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGc                                                              <  1:25975/79‑1 (MQ=255)                                                                                                                            tCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCat  <  1:590511/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                               GCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT  >  NC_000913/2189656‑2189917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑