Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,311,756	A→G	S332P (TCC→CCC)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,311,756	0	A	G	100.0%	117.8 / NA	32	S332P (TCC→CCC)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

TTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACT  >  NC_000913/2311625‑2311890                                                                                                                                    |                                                                                                                                       tttGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGttt                                                                                                                                 >  1:102452/1‑139 (MQ=255)       cGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTttgttg                                                                                                                           >  1:422337/1‑139 (MQ=255)               aTCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAgtagta                                                                                                                   >  1:277156/1‑139 (MQ=255)                        aGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCAcc                                                                                                          <  2:109846/139‑1 (MQ=255)                          aaCTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                                                                        >  2:312641/1‑139 (MQ=255)                               gTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatca                                                                                                   >  1:292740/1‑139 (MQ=255)                                     cAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTATGTTGAAGTAGTAGGTAGCACCAACATCAACatat                                                                                             <  1:188100/139‑1 (MQ=255)                                        aCCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                                                                          <  2:422337/139‑1 (MQ=255)                                         cccAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                                                                                         >  2:614356/1‑139 (MQ=255)                                                  aCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAgg                                                                                       <  2:704037/132‑1 (MQ=255)                                                  aCGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAgg                                                                                       >  1:704037/1‑132 (MQ=255)                                                   cGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGCTGTCGTCCAGCAGGTTGATTTAGTAGTCAACGTAGGTGGGCATGTTTTTGTTGGAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc                                                                               >  2:509316/1‑139 (MQ=255)                                                    gATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg                                                                              <  2:325218/139‑1 (MQ=255)                                                            cAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtc                                                                         <  2:70254/136‑1 (MQ=255)                                                            cAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtc                                                                         >  1:70254/1‑136 (MQ=255)                                                              gtgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc                                                                    >  1:169943/1‑139 (MQ=255)                                                                 ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc                                                                 <  1:548200/139‑1 (MQ=255)                                                                 ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc                                                                 <  1:312641/139‑1 (MQ=255)                                                                   gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAagta                                                                                                                      >  2:189818/1‑84 (MQ=255)                                                                   gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAagta                                                                                                                      <  1:189818/84‑1 (MQ=255)                                                                    aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAc                                                              >  2:214136/1‑139 (MQ=255)                                                                    aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAc                                                              >  2:509776/1‑139 (MQ=255)                                                                                cGAGTGAACTGGCTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCACGTTTTTAc                                                  <  1:509316/139‑1 (MQ=255)                                                                                  aGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt                                                <  2:292740/139‑1 (MQ=255)                                                                                        cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGAc                                          <  2:102452/139‑1 (MQ=255)                                                                                              gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                                                                <  1:602795/79‑1 (MQ=255)                                                                                              gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                                                                >  2:602795/1‑79 (MQ=255)                                                                                                cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                                                                        <  2:398043/69‑1 (MQ=255)                                                                                                cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                                                                        >  1:398043/1‑69 (MQ=255)                                                                                                                          aCGTAGGTGGGTATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt        <  2:277156/139‑1 (MQ=255)                                                                                                                               ggTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc   <  1:214136/139‑1 (MQ=255)                                                                                                                                gTGGGCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt  >  1:363507/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                       TTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACT  >  NC_000913/2311625‑2311890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑