Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,319,725 T→C E51G (GAA→GGA)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,319,7250TC95.3% 138.4 / ‑2.1 43E51G (GAA→GGA) rcsChybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (22/19);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

CGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAA  >  NC_000913/2319605‑2319857
                                                                                                                        |                                                                                                                                    
cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGAtt                                                                                                                    <  2:588910/139‑1 (MQ=255)
             cacCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGATTAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTaa                                                                                                       <  1:488349/139‑1 (MQ=255)
                 gTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCa                                                                                                   <  2:105433/139‑1 (MQ=255)
                    ttCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTa                                                                                                <  2:708777/139‑1 (MQ=255)
                            aTAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTa                                                                                        >  2:760494/1‑139 (MQ=255)
                                              tGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGc                                                                      <  1:58378/139‑1 (MQ=255)
                                              tGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGc                                                                      >  1:401004/1‑139 (MQ=255)
                                                       gCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTaa                                                                                                       >  2:757416/1‑97 (MQ=255)
                                                       gCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTaa                                                                                                       <  1:757416/97‑1 (MQ=255)
                                                        ctctTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGc                                                            >  1:494555/1‑139 (MQ=255)
                                                                     tcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACg                                               >  2:427546/1‑139 (MQ=255)
                                                                           gTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAGACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATg                                         >  2:735509/1‑139 (MQ=255)
                                                                           gTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATg                                         >  1:534084/1‑139 (MQ=255)
                                                                              cGTTGAATAAAGCGCTGTGCCAGATCGGAACTCAGATTAAATCCCTGATGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                      <  1:244698/139‑1 (MQ=255)
                                                                              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTg                                                                                                             <  1:442511/68‑1 (MQ=255)
                                                                              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTg                                                                                                             >  2:442511/1‑68 (MQ=255)
                                                                              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCAc                                                    <  2:275158/125‑1 (MQ=255)
                                                                              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCAc                                                    >  1:275158/1‑125 (MQ=255)
                                                                              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                      >  2:616077/1‑139 (MQ=255)
                                                                              cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                      >  1:468714/1‑139 (MQ=255)
                                                                                ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTg                                    >  1:335269/1‑139 (MQ=255)
                                                                                   aaTAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAAc                                 >  2:392182/1‑139 (MQ=255)
                                                                                               ggCCTGATCCGAACTCAGATTAAATCCCTTACGTATTTCCGACTCTCGCTGATGGATCGGATTAACCATGTAAAAAGCGGATGAAAGAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg                     <  2:300632/138‑1 (MQ=255)
                                                                                               aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAATAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg                     <  2:534084/139‑1 (MQ=255)
                                                                                               aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                      <  2:406075/122‑1 (MQ=255)
                                                                                               aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc                                      >  1:406075/1‑122 (MQ=255)
                                                                                               aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg                     >  2:638750/1‑139 (MQ=255)
                                                                                               aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg                     <  1:427546/139‑1 (MQ=255)
                                                                                               aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg                     <  2:401004/139‑1 (MQ=255)
                                                                                                     aTCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTc               >  1:226910/1‑139 (MQ=255)
                                                                                                       cGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTaa                                                                                                       <  1:677248/49‑1 (MQ=255)
                                                                                                       cGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTaa                                                                                                       >  2:677248/1‑49 (MQ=255)
                                                                                                         gAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAggg           >  1:481647/1‑139 (MQ=255)
                                                                                                         gAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAggg           <  2:616383/139‑1 (MQ=255)
                                                                                                               aGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTaa                                                                                                       <  1:16488/41‑1 (MQ=255)
                                                                                                               aGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTaa                                                                                                       >  2:16488/1‑41 (MQ=255)
                                                                                                               aGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc                                        >  2:191830/1‑104 (MQ=255)
                                                                                                               aGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc                                        <  1:191830/104‑1 (MQ=255)
                                                                                                               aGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCa              <  2:107688/130‑1 (MQ=255)
                                                                                                               aGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCa              >  1:107688/1‑130 (MQ=255)
                                                                                                                 aTTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGTGCGAGGCttt                >  1:688141/1‑126 (MQ=255)
                                                                                                                 aTTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCttt                <  2:688141/126‑1 (MQ=255)
                                                                                                                 aTTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATG‑AAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaa  >  2:540314/1‑139 (MQ=255)
                                                                                                                        |                                                                                                                                    
CGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAA  >  NC_000913/2319605‑2319857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑