Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,523,413	T→C	T191T (ACA→ACG)	xapB ←	xanthosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,523,413	0	T	C	100.0%	120.3 / NA	32	T191T (ACA→ACG)	xapB	xanthosine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

GTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGATGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGATATACAGCTGCAGACTACTCAATTCCAGATGCAGCAGGCTTACTGCCCACATC  >  NC_000913/2523280‑2523548                                                                                                                                      |                                                                                                                                        gTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCATGCTGGCAAGCGACGTGGt                                                                                                                                    >  1:390673/1‑139 (MQ=255)       aCGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCttt                                                                                                                              >  1:602390/1‑139 (MQ=255)          gATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCtttttt                                                                                                                           >  1:249877/1‑139 (MQ=255)             tACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTtctc                                                                                                                        >  2:557176/1‑139 (MQ=255)                cAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGc                                                                                                                     >  2:86808/1‑139 (MQ=255)                     aCGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCg                                                                                                                >  1:629061/1‑139 (MQ=255)                           gTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCt                                                                                                          <  2:602390/139‑1 (MQ=255)                                     gTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGt                                                                                                >  2:37766/1‑139 (MQ=255)                                        ccGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAg                                                                                             <  1:657242/139‑1 (MQ=255)                                        ccGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAg                                                                                             >  2:425638/1‑139 (MQ=255)                                           cACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGt                                                                                                >  1:695395/1‑133 (MQ=255)                                           cACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGt                                                                                                <  2:695395/133‑1 (MQ=255)                                           cACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAgcgc                                                                                          >  1:563236/1‑139 (MQ=255)                                           cACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAgcgc                                                                                          >  1:738918/1‑139 (MQ=255)                                           cACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGAACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAgcgc                                                                                          >  1:633152/1‑139 (MQ=255)                                                aGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGACAAAGTCAGCGCATAAg                                                                                     >  1:706465/1‑139 (MQ=255)                                                          cAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGACAAAGTCAGCGCATAAGCCGAcagcag                                                                           <  2:706465/139‑1 (MQ=255)                                                           aaaGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAACAGACagcagc                                                                          >  1:754274/1‑139 (MQ=255)                                                                ggAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGa                                                                     <  1:37766/139‑1 (MQ=255)                                                                    aaaGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCa                                                                 <  2:629061/139‑1 (MQ=255)                                                                        aTGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCgg                                                             <  2:249877/139‑1 (MQ=255)                                                                        aTGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCgg                                                             <  2:134868/139‑1 (MQ=255)                                                                                  gTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGAtata                                                   <  2:326511/139‑1 (MQ=255)                                                                                                 gCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGATATACAGCTGCAGactact                                    <  2:563236/139‑1 (MQ=255)                                                                                                  cACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCgg                                                             <  1:711641/113‑1 (MQ=255)                                                                                                  cACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCgg                                                             >  2:711641/1‑113 (MQ=255)                                                                                                          cATCCAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGATATACAGCTGCAGACTACTCAATTCCAg                           <  1:86808/139‑1 (MQ=255)                                                                                                              cAGACCCAGCTTGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGATATACAGCTGCAGACTACTCAATTCCAGATgc                       <  2:738918/139‑1 (MQ=255)                                                                                                                         tGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGAcg                                                          >  2:206561/1‑93 (MQ=255)                                                                                                                         tGCTGGCAAGCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGAcg                                                          <  1:206561/93‑1 (MQ=255)                                                                                                                                  gCGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGATATACAGCTGCAGACTACTCAATTCCAGATGCAGCAGGCTTACTGCCCACAt   <  2:390673/139‑1 (MQ=255)                                                                                                                                   cGACGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGATATACAGCTGCAGACTACTCAATTCCAGATGCAGCAGGCTTACTGCCCACATc  >  1:591018/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        GTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATGGCCATGCGTGGATTTTTAAACAGCACGAAGGCATCCAGACCCAGCTTGCTGGCAAGCGATGTGGTCGCTTTTTTCTCCGCAACCGGAATCTTCGGCAAAGTCAGCGCATAAGCCGACAGCAGCAATGACGCACCGGACGCGATATACAGCTGCAGACTACTCAATTCCAGATGCAGCAGGCTTACTGCCCACATC  >  NC_000913/2523280‑2523548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑