Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	324,641	C→T	intergenic (+188/+55)	ykgG → / ← ykgH	LutC family protein, putative electron transport chain YkgEFG component/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	324,641	0	C	T	100.0%	194.0 / NA	55	intergenic (+188/+55)	ykgG/ykgH	LutC family protein, putative electron transport chain YkgEFG component/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/27);  total (28/27)

AACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCATTTTTTTGACAAGGCAAAACATTACAGAAATTA  >  NC_000913/324519‑324763                                                                                                                           |                                                                                                                           aaCCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACtt                                                                                                            >  1:105139/1‑139 (MQ=255) aaCCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACtt                                                                                                            <  1:187146/139‑1 (MQ=255) aaCCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACtt                                                                                                            <  2:105139/139‑1 (MQ=255)   ccTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCa                                                                                                          >  1:757415/1‑139 (MQ=255)      gACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCt                                                                                                       >  1:3545/1‑139 (MQ=255)       aCTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCtt                                                                                                      <  1:89342/139‑1 (MQ=255)             ccccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACa                                                                                                >  1:705610/1‑139 (MQ=255)               ccGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAgg                                                                                              <  2:757415/139‑1 (MQ=255)                 gTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCa                                                                                            <  2:705610/139‑1 (MQ=255)                   tCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                                                          <  2:438023/139‑1 (MQ=255)                     cGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCActct                                                                                        <  2:3545/139‑1 (MQ=255)                                     ctctTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                                                          >  2:278227/1‑121 (MQ=255)                                     ctctTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                                                          <  1:278227/121‑1 (MQ=255)                                     ctctTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATgttgtt                                                                        >  2:755181/1‑139 (MQ=255)                                                  atCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                                                          >  1:467888/1‑108 (MQ=255)                                                  atCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                                                          <  2:467888/108‑1 (MQ=255)                                                    cccggTATTACCAATGCCAGGATATTTACCGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAgg                                                                                              <  1:476599/99‑1 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTc                                                                                                           <  1:467981/89‑1 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTc                                                                                                           >  2:467981/1‑89 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAgg                                                                                              >  2:476599/1‑102 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCa                                                                                            >  2:629583/1‑104 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCa                                                                                            <  1:629583/104‑1 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGcg                                                              <  2:488616/134‑2 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGcc                                                              >  1:488616/1‑134 (MQ=255)                                                    ccTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCagag                                                         >  2:649869/1‑139 (MQ=255)                                                      tGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGtt                                                       <  1:755181/139‑1 (MQ=255)                                                       ggTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGttt                                                      >  1:627623/1‑139 (MQ=255)                                                          aTTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTttgt                                                   <  1:77575/139‑1 (MQ=255)                                                           ttACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCa                                                                                            <  2:592047/97‑1 (MQ=255)                                                           ttACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCa                                                                                            >  1:592047/1‑97 (MQ=255)                                                                  tGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACtt                                                                                                            >  2:408080/1‑74 (MQ=255)                                                                  tGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACtt                                                                                                            <  1:408080/74‑1 (MQ=255)                                                                  tGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                                                          <  1:302297/92‑1 (MQ=255)                                                                  tGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCAct                                                                                          >  2:302297/1‑92 (MQ=255)                                                                  tGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTc                                           >  2:40592/1‑139 (MQ=255)                                                                  tGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGACATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCGCAGAGTTTTGTTGGTATTc                                           >  2:511313/1‑139 (MQ=255)                                                                        gATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtg                                                                             >  2:3416/1‑99 (MQ=255)                                                                        gATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAAtg                                                                             <  1:3416/99‑1 (MQ=255)                                                                        gATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCag                                                           >  2:194059/1‑117 (MQ=255)                                                                        gATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCag                                                           <  1:194059/117‑1 (MQ=255)                                                                                   tGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCt                                                                                                       >  1:508310/1‑62 (MQ=255)                                                                                   tGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCt                                                                                                       <  2:508310/62‑1 (MQ=255)                                                                                   tGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCATTTTTTTGa                          >  1:56324/1‑139 (MQ=255)                                                                                    gAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTc                                                                                                                      >  2:436843/1‑46 (MQ=255)                                                                                    gAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTc                                                                                                                      <  1:436843/46‑1 (MQ=255)                                                                                    gAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAgg                                                                                              <  2:642477/70‑1 (MQ=255)                                                                                    gAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAgg                                                                                              >  1:642477/1‑70 (MQ=255)                                                                                             aGGCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCATTTTTTTGACAAGGCaaaa                <  1:511313/139‑1 (MQ=255)                                                                                               gCATCCATTCCTGAAAACGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCATTTTTTTGACAAGGCAAAACa              >  1:689226/1‑139 (MQ=255)                                                                                                            aaaaCGGGTTCCCTTTCCGGTCATTGTCACTTCAGCtt                                                                                                      >  2:54234/1‑38 (MQ=255)                                                                                                            aaaaCGGGTTCCCTTTCCGGTCATTGTCACTTCAGCtt                                                                                                      <  1:54234/38‑1 (MQ=255)                                                                                                             aaaCGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGcc                                                              <  2:130133/77‑1 (MQ=255)                                                                                                             aaaCGGGTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGcc                                                              >  1:130133/1‑77 (MQ=255)                                                                                                                 gggTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCATTTTTTTGACAAGGCAAAACATTACAGAAATTa  <  2:260118/133‑1 (MQ=255)                                                                                                                 gggTTCCCTTTCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCATTTTTTTGACAAGGCAAAACATTACAGAAATTa  >  1:260118/1‑133 (MQ=255)                                                                                                                           |                                                                                                                           AACCTGACTTAACCCCGTTCCGTCATGGTTATGCTGCTCTTCTGTAAATATCCTGGTATTACCAATGCCAGGATATTTACAGTGAAAAAGACAGGCATCCATTCCTGAAAACGGGTTCCCTTCCCGGTCATTGTCACTTCAGCTTTATACAGGCACTCTCTATCAGAATGTTGTTTATTACTGCCCAGAGTTTTGTTGGTATTCATCTACCATTTTTTTGACAAGGCAAAACATTACAGAAATTA  >  NC_000913/324519‑324763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑