Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,051,507 G→A L271L (CTG→TTG)  ubiI ← 2‑octaprenylphenol hydroxylase, FAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,051,5070GA100.0% 77.3 / NA 23L271L (CTG→TTG) ubiI2‑octaprenylphenol hydroxylase, FAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

TTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGA  >  NC_000913/3051373‑3051641
                                                                                                                                      |                                                                                                                                      
ttCTGCAATCACCTCTGCAACACCCATAAGACCGAGATTTACCCCCGGCCCCGCCAGCGGGTTAATGGTATGTGCGTCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATggg                                                                                                                                    <  2:513034/139‑1 (MQ=255)
                      tCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCaa                                                                                                              >  1:226075/1‑139 (MQ=255)
                             aGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCa                                                                                                       <  2:704875/139‑1 (MQ=255)
                                    aTTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGccc                                                                                                >  1:673977/1‑139 (MQ=255)
                                                 cccGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa                                                                                   <  1:16167/139‑1 (MQ=255)
                                                               aaTGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa                                                                                   <  1:510877/125‑1 (MQ=255)
                                                               aaTGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa                                                                                   >  2:510877/1‑125 (MQ=255)
                                                                      tgtgCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATggg                                                                                                                                    <  1:665084/69‑1 (MQ=255)
                                                                      tgtgCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATggg                                                                                                                                    >  2:665084/1‑69 (MQ=255)
                                                                         gcggcgTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTaa                                                           >  2:692842/1‑139 (MQ=255)
                                                                             cgTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTc                                                       >  1:78449/1‑139 (MQ=255)
                                                                             cgTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTc                                                       >  1:208422/1‑139 (MQ=255)
                                                                               tCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGt                                                     <  2:171187/139‑1 (MQ=255)
                                                                                  cccACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCtt                                                  >  2:531087/1‑139 (MQ=255)
                                                                                         gcgcCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGGTTAAATTCGTCTTCACCTGc                                           <  1:531087/139‑1 (MQ=255)
                                                                                               gACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCctgctg                                     >  2:391323/1‑139 (MQ=255)
                                                                                                    tGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATcc                                >  2:138299/1‑139 (MQ=255)
                                                                                                     gCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCg                               <  1:138299/139‑1 (MQ=255)
                                                                                                          gCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTgcg                          >  1:67842/1‑139 (MQ=255)
                                                                                                                   cgcgcATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCAt                                                                                                      <  2:301496/54‑1 (MQ=255)
                                                                                                                   cgcgcATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCAt                                                                                                      >  1:301496/1‑54 (MQ=255)
                                                                                                                         tAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACa           <  1:211836/139‑1 (MQ=255)
                                                                                                                                  gTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGa  <  2:67842/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGA  >  NC_000913/3051373‑3051641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: