Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	352,986	A→G	N94S (AAC→AGC)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	352,986	0	A	G	100.0%	80.6 / NA	22	N94S (AAC→AGC)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

GCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACT  >  NC_000913/352851‑353111                                                                                                                                        |                                                                                                                              gCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAgcgc                                                                                                                            >  2:46699/1‑139 (MQ=255)           cTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCa                                                                                                                  >  1:467245/1‑139 (MQ=255)                gCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGtt                                                                                                             <  1:46699/139‑1 (MQ=255)                 ccACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTg                                                                                                            <  2:688942/139‑1 (MQ=255)                           aTCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTgg                                                                                                  >  1:668731/1‑139 (MQ=255)                                                     agagGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTg                                                                        <  2:633289/139‑1 (MQ=255)                                                                          cGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTc                                                                                                       >  1:107125/1‑87 (MQ=255)                                                                          cGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTc                                                                                                       <  2:107125/87‑1 (MQ=255)                                                                          cGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGcc                                                   >  2:6271/1‑139 (MQ=255)                                                                           gTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAGTGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCg                                                  <  2:692709/139‑1 (MQ=255)                                                                               ctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTg                                                                                                            >  1:199228/1‑77 (MQ=255)                                                                               ctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTg                                                                                                            <  2:199228/77‑1 (MQ=255)                                                                               ctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTg                                                                           <  2:496776/110‑1 (MQ=255)                                                                               ctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAgc                                              <  2:467245/139‑1 (MQ=255)                                                                               ctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGAGTGCAGCGCGGCGATCGGGTg                                                                           >  1:496776/1‑110 (MQ=255)                                                                                 tCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAg                                               <  1:129287/136‑1 (MQ=255)                                                                                 tCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAg                                               >  2:129287/1‑136 (MQ=255)                                                                                                   cgcACCTTTAGCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCt                          >  2:708701/1‑139 (MQ=255)                                                                                                                  cGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGgcg           >  2:228025/1‑139 (MQ=255)                                                                                                                   gTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGgcgc          <  2:668731/139‑1 (MQ=255)                                                                                                                    tCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCt         >  2:535385/1‑139 (MQ=255)                                                                                                                       gCTGCAGGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTAtt      <  1:535385/139‑1 (MQ=255)                                                                                                                           cATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACt  <  1:6271/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                              GCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACT  >  NC_000913/352851‑353111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑