Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,216,464 G→A R10C (CGC→TGC)  yqjH ← putative siderophore interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,216,4640GA100.0% 95.7 / NA 28R10C (CGC→TGC) yqjHputative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

ACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCT  >  NC_000913/3216348‑3216597
                                                                                                                    |                                                                                                                                     
aCGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTa                                                                                                                 <  2:667193/139‑1 (MQ=255)
               gTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGcc                                                                                                  <  1:277873/139‑1 (MQ=255)
                tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCt                                                                                                 >  1:684230/1‑139 (MQ=255)
                tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCt                                                                                                 >  1:162793/1‑139 (MQ=255)
                     cgcCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTg                                                                                            <  1:56715/139‑1 (MQ=255)
                          cgccgcCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCg                                                                                                                           >  1:399595/1‑103 (MQ=255)
                          cgccgcCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCg                                                                                                                           <  2:399595/103‑1 (MQ=255)
                             cgccgAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt                                                                                    <  2:36615/139‑1 (MQ=255)
                                    gACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa                                                                                     >  2:509115/1‑131 (MQ=255)
                                    gACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa                                                                                     <  1:509115/131‑1 (MQ=255)
                                              tGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa                                                                                     <  2:475546/121‑1 (MQ=255)
                                              tGAAAACCGGCGCTCATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACAGCGGGTAGCGGGGGGTGTTATTCATTATTATCGCCTTCGTGATGGTaa                                                                                     >  1:475546/1‑121 (MQ=255)
                                                     cGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTTATGGTAATCAGATATATCTAAATAAAACTCGc                                                            <  2:466027/139‑1 (MQ=255)
                                                     cGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc                                                            >  1:453145/1‑139 (MQ=255)
                                                                                 agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt                                                                                    <  1:169078/87‑1 (MQ=255)
                                                                                 agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt                                                                                    >  2:169078/1‑87 (MQ=255)
                                                                                 agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc                                                            <  2:131355/111‑1 (MQ=255)
                                                                                 agtcagtTCACGGAAGCGCAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc                                                            >  1:131355/1‑111 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGa                                                                                           <  1:658879/64‑1 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGa                                                                                           >  2:658879/1‑64 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGAt                     <  2:261159/134‑1 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGAt                     >  1:261159/1‑134 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa                >  1:534382/1‑139 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa                >  2:495805/1‑139 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa                >  2:498957/1‑139 (MQ=255)
                                                                                                 cgcAGATCATTGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCACATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa                <  2:534382/139‑1 (MQ=255)
                                                                                                          ttGCGAACGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAGCTGata       <  1:498957/139‑1 (MQ=255)
                                                                                                               aaCGCACTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCt  <  1:649924/139‑1 (MQ=255)
                                                                                                                    |                                                                                                                                     
ACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCT  >  NC_000913/3216348‑3216597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: