Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	365,380	A→G	L309P (CTC→CCC)	lacZ ←	beta‑D‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,380	0	A	G	100.0%	84.7 / NA	23	L309P (CTC→CCC)	lacZ	beta‑D‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

GGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGC  >  NC_000913/365260‑365501                                                                                                                         |                                                                                                                          ggCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGgcgc                                                                                                         <  1:267776/139‑1 (MQ=255)  gCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCt                                                                                                        >  2:635154/1‑139 (MQ=255)                                       cACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGtgtg                                                                   >  1:446064/1‑139 (MQ=255)                                       cACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGtgtg                                                                   >  1:554331/1‑139 (MQ=255)                                            cgcgGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg                                                              <  2:776899/139‑1 (MQ=255)                                            cgcgGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg                                                              <  2:339650/139‑1 (MQ=255)                                              cgGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGAt                                                            <  2:511486/139‑1 (MQ=255)                                                    ccGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCAt                                                      <  2:554331/139‑1 (MQ=255)                                                    ccGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCAt                                                      <  2:446064/139‑1 (MQ=255)                                                       aCATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                                                                                >  1:469695/1‑110 (MQ=255)                                                       aCATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                                                                                <  2:469695/110‑1 (MQ=255)                                                              aGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACg                                            <  1:206019/139‑1 (MQ=255)                                                                            aTCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                                                                                <  2:414246/89‑1 (MQ=255)                                                                            aTCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                                                                                >  1:414246/1‑89 (MQ=255)                                                                            aTCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAAttt                              >  1:516998/1‑139 (MQ=255)                                                                                gCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCAcc                          <  2:516998/139‑1 (MQ=255)                                                                                 cGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCAccg                         <  1:635154/139‑1 (MQ=255)                                                                                 cGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCAccg                         >  2:496693/1‑139 (MQ=255)                                                                                                 tgCAGTTCAACCACCGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGc         >  1:324801/1‑139 (MQ=255)                                                                                                              ccGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg                                                              >  1:99613/1‑73 (MQ=255)                                                                                                              ccGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg                                                              <  2:99613/73‑1 (MQ=255)                                                                                                              ccGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGc  <  2:237237/133‑1 (MQ=255)                                                                                                              ccGCACGATAGGGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGc  >  1:237237/1‑133 (MQ=255)                                                                                                                         |                                                                                                                          GGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGC  >  NC_000913/365260‑365501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑