Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,136,860 A→G T251T (ACA→ACG yijE → EamA‑like transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,136,8600AG100.0% 91.3 / NA 26T251T (ACA→ACGyijEEamA‑like transporter family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

GTGTTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC  >  NC_000913/4136753‑4136995
                                                                                                           |                                                                                                                                       
gtgtTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCt                                                                                                                                       >  2:631751/1‑110 (MQ=255)
gtgtTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCt                                                                                                                                       <  1:631751/110‑1 (MQ=255)
         gcgcTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGgc                                                                                                 >  1:317210/1‑139 (MQ=255)
                         cGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATcc                                                                                 >  2:79960/1‑139 (MQ=255)
                         cGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATcc                                                                                 >  2:729319/1‑139 (MQ=255)
                         cGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATcc                                                                                 >  1:733602/1‑139 (MQ=255)
                                      ggCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGaa                                                                    >  1:201636/1‑139 (MQ=255)
                                           tGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAg                                                               <  2:317210/139‑1 (MQ=255)
                                             gCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCg                                                             <  2:607084/139‑1 (MQ=255)
                                                tGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTTGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTa                                                          <  1:680769/139‑1 (MQ=255)
                                                       tATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGtt                                                                                                                                >  2:678850/1‑62 (MQ=255)
                                                       tATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGtt                                                                                                                                <  1:678850/62‑1 (MQ=255)
                                                          ggTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTattgtgctgat                                                <  2:201636/139‑1 (MQ=255)
                                                               tttgtATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg                                                                            <  1:763715/106‑1 (MQ=255)
                                                               tttgtATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg                                                                            >  2:763715/1‑106 (MQ=255)
                                                                     ttGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                     <  1:729319/139‑1 (MQ=255)
                                                                          aaaCTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg                                                                            <  1:256601/95‑1 (MQ=255)
                                                                          aaaCTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg                                                                            >  2:256601/1‑95 (MQ=255)
                                                                                              ccAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa            >  1:522709/1‑139 (MQ=255)
                                                                                                  cTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAg                                                               >  2:402124/1‑84 (MQ=255)
                                                                                                  cTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAg                                                               <  1:402124/84‑1 (MQ=255)
                                                                                                  cTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa        <  2:201891/139‑1 (MQ=255)
                                                                                                   ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                  <  1:715343/112‑1 (MQ=255)
                                                                                                   ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                  >  2:715343/1‑112 (MQ=255)
                                                                                                   ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga         >  2:588374/1‑137 (MQ=255)
                                                                                                   ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga         <  1:588374/137‑1 (MQ=255)
                                                                                                       gCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGACGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt   <  2:522709/139‑1 (MQ=255)
                                                                                                        cacgCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc  <  2:733602/139‑1 (MQ=255)
                                                                                                           |                                                                                                                                       
GTGTTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC  >  NC_000913/4136753‑4136995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: