Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,296	T→A	I316N (ATC→AAC)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,296	0	T	A	100.0%	111.9 / NA	33	I316N (ATC→AAC)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/16);  total (17/16)

CGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT  >  NC_000913/4186160‑4186423                                                                                                                                         |                                                                                                                                cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACa                                                                                                                               >  1:14852/1‑139 (MQ=255)  gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac                                                                                                                              <  1:336506/139‑1 (MQ=255)  gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac                                                                                                                              <  2:485493/139‑1 (MQ=255)   cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac                                                                                                                              <  2:328345/138‑1 (MQ=255)   cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac                                                                                                                              >  1:328345/1‑138 (MQ=255)                aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGc                                                                                                                >  1:441446/1‑139 (MQ=255)                                   tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                             >  1:401930/1‑139 (MQ=255)                                   tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                                                             >  1:624852/1‑139 (MQ=255)                                       ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                         <  2:401930/139‑1 (MQ=255)                                       ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc                                                                                         <  2:14852/139‑1 (MQ=255)                                          tCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATg                                                                                      >  1:350440/1‑139 (MQ=255)                                               cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa                                                                                 >  2:575294/1‑139 (MQ=255)                                                                 tacgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGttt                                                               >  2:463723/1‑139 (MQ=255)                                                                    gtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCg                                                            >  1:72659/1‑139 (MQ=255)                                                                        cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                                                        >  1:328300/1‑139 (MQ=255)                                                                              aCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGCTCTAACAAGCGTCCTCTGAAATCTTTGGCCGTCATGATCAAAGGTAAACAGGGTCGTGTCCGTCAGAACCTg                                                  <  1:476830/139‑1 (MQ=255)                                                                                    gctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGt                                                                                                                          <  2:755806/61‑1 (MQ=255)                                                                                    gctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGt                                                                                                                          >  1:755806/1‑61 (MQ=255)                                                                                     ctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa                                           <  2:441446/139‑1 (MQ=255)                                                                                           gAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgtg                                     <  2:624852/139‑1 (MQ=255)                                                                                             aGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGACCTCTGAAATCTTTGGCCGACATGATCAAAGATAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGtt                                   >  2:509487/1‑139 (MQ=255)                                                                                                                 aTAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    >  1:650358/1‑70 (MQ=255)                                                                                                                 aTAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    <  2:650358/70‑1 (MQ=255)                                                                                                                  tAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa              <  1:575294/139‑1 (MQ=255)                                                                                                                    aCGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt            <  2:328300/139‑1 (MQ=255)                                                                                                                     cGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTc                                               >  2:355286/1‑103 (MQ=255)                                                                                                                     cGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTc                                               <  1:355286/103‑1 (MQ=255)                                                                                                                       gtcgtcGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGactac                             <  2:11660/119‑1 (MQ=255)                                                                                                                       gtcgtcGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGactac                             >  1:11660/1‑119 (MQ=255)                                                                                                                       gtcgtcGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCAc         >  2:269175/1‑139 (MQ=255)                                                                                                                          gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt                                      <  1:763213/107‑1 (MQ=255)                                                                                                                          gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt                                      >  2:763213/1‑107 (MQ=255)                                                                                                                          gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  1:5752/139‑1 (MQ=255)                                                                                                                          gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt      <  2:72659/139‑1 (MQ=255)                                                                                                                              cgGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGTTCGTTCTGTAATCACCGTAGGt  <  2:350440/139‑1 (MQ=255)                                                                                                                                    tGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    >  2:376640/1‑51 (MQ=255)                                                                                                                                    tGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt                                                                                    <  1:376640/51‑1 (MQ=255)                                                                                                                                         |                                                                                                                                CGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT  >  NC_000913/4186160‑4186423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑