Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,385,643	T→C	L84S (TTA→TCA)	yjeN →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,385,643	0	T	C	100.0%	123.7 / NA	34	L84S (TTA→TCA)	yjeN	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)

TAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATC  >  NC_000913/4385539‑4385769                                                                                                         |                                                                                                                               tAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCtat                                                                                              >  2:681121/1‑139 (MQ=255)    cACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGc                                                                                           >  1:498650/1‑139 (MQ=255)    cACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGc                                                                                           >  2:185506/1‑139 (MQ=255)    cACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGc                                                                                           >  1:346591/1‑139 (MQ=255)      ccATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGa                                                                                         <  2:498650/139‑1 (MQ=255)              acCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGc                                                                                 <  2:346591/139‑1 (MQ=255)                  tCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAcc                                                                             >  2:463714/1‑139 (MQ=255)                    aaaGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCgg                                                                           >  1:756360/1‑139 (MQ=255)                     aaGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGt                                                                          <  1:442241/139‑1 (MQ=255)                     aaGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATGTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGt                                                                          <  1:539281/139‑1 (MQ=255)                          cgcgATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCt                                                                     >  1:396747/1‑139 (MQ=255)                              aTGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGc                                                                                 <  2:384242/123‑1 (MQ=255)                              aTGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGc                                                                                 >  1:384242/1‑123 (MQ=255)                              aTGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATg                                                                 <  2:143564/139‑1 (MQ=255)                               tGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGa                                                                <  1:681121/139‑1 (MQ=255)                                ggTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAg                                                               <  1:463714/139‑1 (MQ=255)                                 gTAAACGGTGGTGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGt                                                              <  1:185506/139‑1 (MQ=255)                                 gTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGt                                                              <  2:756360/139‑1 (MQ=255)                                         tGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATg                                                                                                    <  1:369929/93‑1 (MQ=255)                                         tGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATg                                                                                                    >  2:369929/1‑93 (MQ=255)                                         tGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCAt                                                      >  1:335200/1‑139 (MQ=255)                                           gggAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                    <  2:335200/139‑1 (MQ=255)                                                                     cttatcttatCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCACTATAGCGAAATTAAGCGACCGGt                                                                          <  2:529675/91‑1 (MQ=255)                                                                     cttatcttagCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGAATTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGt                                                                          >  1:529675/1‑91 (MQ=255)                                                                           ttatcCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCg                    <  2:615895/139‑1 (MQ=255)                                                                                  aTGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                    <  2:187873/100‑1 (MQ=255)                                                                                  aTGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                    >  1:187873/1‑100 (MQ=255)                                                                                  aTGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCctct             >  2:333135/1‑139 (MQ=255)                                                                                    gTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTgg           >  1:185944/1‑139 (MQ=255)                                                                                     tCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGa          <  2:185944/139‑1 (MQ=255)                                                                                       tGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGGGCGCATGTTTGTc                                               <  2:107845/100‑1 (MQ=255)                                                                                       tGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGGGCGCATGTTTGTc                                               >  1:107845/1‑100 (MQ=255)                                                                                         gTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAAc      >  1:160593/1‑139 (MQ=255)                                                                                             ttCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATc  >  2:588240/1‑139 (MQ=255)                                                                                                         |                                                                                                                               TAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATC  >  NC_000913/4385539‑4385769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑