Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,457,798	T→C	E23G (GAA→GGA)	yjgA ←	ribosome‑associated UPF0307 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,457,798	0	T	C	100.0%	138.5 / NA	37	E23G (GAA→GGA)	yjgA	ribosome‑associated UPF0307 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/20);  total (17/20)

CGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGG  >  NC_000913/4457673‑4457924                                                                                                                              |                                                                                                                               cgcgcAGATCCGCATCTAACGGGATCTTATCCAGCGCGTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCAtcgtcttcgtc                                                                                                                   >  2:605121/1‑139 (MQ=255)  gcgcAGATCCGCATCTAACGGGATCTTATCCAGCGCGTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCAtcgtcttcgtct                                                                                                                  <  1:718863/139‑1 (MQ=255)                              tCCAGCGCGTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCAtcgtcttcgt                                                                                                                    >  2:62914/1‑109 (MQ=255)                              tCCAGCGCGTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCAtcgtcttcgt                                                                                                                    <  1:62914/109‑1 (MQ=255)                                   cgcgTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTTGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAg                                                                                 >  1:669657/1‑139 (MQ=255)                                   cgcgTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAg                                                                                 >  1:732978/1‑139 (MQ=255)                                   cgcgTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAg                                                                                 >  2:558605/1‑139 (MQ=255)                                    gcgTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGt                                                                                <  1:558605/139‑1 (MQ=255)                                       tttttCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGtca                                                                                                         <  2:605316/111‑1 (MQ=255)                                       tttttCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGtca                                                                                                         >  1:605316/1‑111 (MQ=255)                                       tttttCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCtt                                                                             >  1:51666/1‑139 (MQ=255)                                        ttttCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTc                                                                            >  2:322041/1‑139 (MQ=255)                                               cAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGgctgc                                                                     <  2:732978/139‑1 (MQ=255)                                                   tCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAg                                                                 <  2:51666/139‑1 (MQ=255)                                                   tCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAg                                                                 <  1:560551/139‑1 (MQ=255)                                                           ttCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCAtcgtctt                                                                                                                       >  1:779009/1‑77 (MQ=255)                                                           ttCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCAtcgtctt                                                                                                                       <  2:779009/77‑1 (MQ=255)                                                            tCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAg                                                        >  1:189809/1‑139 (MQ=255)                                                             ccGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTTGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAgg                                                       <  2:669657/139‑1 (MQ=255)                                                              cgcgCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGc                                                      <  1:322041/139‑1 (MQ=255)                                                                cgcCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTc                                                    <  2:300/139‑1 (MQ=255)                                                                 gcCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTc                                                    >  1:381145/1‑138 (MQ=255)                                                                 gcCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTc                                                    <  2:381145/138‑1 (MQ=255)                                                                              aGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAgg                                      <  1:212050/139‑1 (MQ=255)                                                                                 tcctcGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCg                                                                           >  1:69942/1‑99 (MQ=255)                                                                                 tcctcGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCg                                                                           <  2:69942/99‑1 (MQ=255)                                                                                      ggCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGtt                              <  2:619432/139‑1 (MQ=255)                                                                                      ggCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGtt                              <  2:189809/139‑1 (MQ=255)                                                                                         aTCACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTAcc                           <  2:541159/139‑1 (MQ=255)                                                                                           cACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAgg                                                       >  2:652112/1‑109 (MQ=255)                                                                                           cACGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAgg                                                       <  1:652112/109‑1 (MQ=255)                                                                                             cGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCgg                                                                          <  2:343087/88‑1 (MQ=255)                                                                                             cGTTTAATTTCACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCgg                                                                          >  1:343087/1‑88 (MQ=255)                                                                                                       cACTTTTACTGACCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCa             >  2:81658/1‑139 (MQ=255)                                                                                                                  aCCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAgg                                      >  2:579232/1‑103 (MQ=255)                                                                                                                  aCCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAgg                                      <  1:579232/103‑1 (MQ=255)                                                                                                                  aCCCAGATAATTCCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACgg  >  1:243306/1‑139 (MQ=255)                                                                                                                              |                                                                                                                               CGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGTTTTTCCCCAGATCAACAATTTCCGCGCCAAGGCGTTTTAGCTCCTCGGCATCACGTTTAATTTCACTTTTACTGACCCAGATAATTTCATCGTCTTCGTCTTCGATGTCATCACCGGGAACGTCGTCGAGCCAGTCTTCGGGCTGCTTAGTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGG  >  NC_000913/4457673‑4457924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑