Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,366	(A)7→6	coding (178/333 nt)	yegP →	UPF0339 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,360	0	A	.	100.0%	90.4 / NA	22	coding (172/333 nt)	yegP	UPF0339 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/10);  total (12/10)

GATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAA  >  NC_000913/2165222‑2165487                                                                                                                                           |                                                                                                                                gATAATCAGTTCCGGTGTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGACGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATgaga                                                                                                                                 >  1:724354/1‑139 (MQ=255)      tCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGaaaaaa                                                                                                                            <  2:540706/139‑1 (MQ=255)          ttCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGaaaaaacggc                                                                                                                        >  2:188619/1‑135 (MQ=255)                    tgCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGc                                                                                                             >  2:219128/1‑139 (MQ=255)                    tgCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGc                                                                                                             >  1:630402/1‑139 (MQ=255)                                              aTCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGc                                                                                   <  2:240983/139‑1 (MQ=255)                                                         cGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCaaa                                                                        >  2:171653/1‑139 (MQ=255)                                                           aGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAAtt                                                                      >  2:790846/1‑139 (MQ=255)                                                                  taCCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCt                                                               <  1:188619/139‑1 (MQ=255)                                                                  taCCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCt                                                               <  1:290202/139‑1 (MQ=255)                                                                       cAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCt                                                               >  2:534658/1‑134 (MQ=255)                                                                       cAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCt                                                               <  1:534658/134‑1 (MQ=255)                                                                          aaaCCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTccagccag                                                       <  2:630402/139‑1 (MQ=255)                                                                               tctGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGTCAGATGTa                                                  >  2:629363/1‑139 (MQ=255)                                                                                  gCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGc                                               <  1:219128/139‑1 (MQ=255)                                                                                                cgcgTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAAtctc                                 >  2:39094/1‑139 (MQ=255)                                                                                                  cgTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGTAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTccagcc                                                         <  1:62663/113‑1 (MQ=255)                                                                                                  cgTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTccagcc                                                         >  2:62663/1‑113 (MQ=255)                                                                                                        tGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAAcc                         >  1:664295/1‑139 (MQ=255)                                                                                                             aGCAACAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAAt                    <  1:790846/139‑1 (MQ=255)                                                                                                                  cAGCCCGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCt               <  2:664295/139‑1 (MQ=255)                                                                                                                     cccGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCg            >  2:100438/1‑139 (MQ=255)                                                                                                                       cGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGtt          >  2:654759/1‑139 (MQ=255)                                                                                                                       cGCAAGAAGAACGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGtt          >  2:685110/1‑139 (MQ=255)                                                                                                                               gaaCGCTATGAG‑AAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCaa  <  1:39094/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                GATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAA  >  NC_000913/2165222‑2165487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑