Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,017	(C)7→8	coding (200/2019 nt)	hyfB →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,010	1	.	C	100.0%	166.9 / NA	45	A65P (GCC→CCC)	hyfB	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (20/25);  total (20/25)

GCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAC  >  NC_000913/2601875‑2602135                                                                                                                                         |                                                                                                                               gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccc                                                                                                                            >  2:339442/1‑139 (MQ=255)   ttCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                          <  1:547323/139‑1 (MQ=255)                    gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCg                                                                                                          >  1:789190/1‑139 (MQ=255)                            aGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAAcc                                                                                                  <  1:480271/139‑1 (MQ=255)                              aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                                >  1:651551/1‑139 (MQ=255)                              aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                                >  2:759033/1‑139 (MQ=255)                              aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                                >  2:545791/1‑139 (MQ=255)                              aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                                >  2:204950/1‑139 (MQ=255)                              aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACACAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                                >  2:219440/1‑139 (MQ=255)                               cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                               >  2:650351/1‑139 (MQ=255)                                   cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                           >  2:705083/1‑139 (MQ=255)                                                cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                              <  2:651551/139‑1 (MQ=255)                                                 ggCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCg                                                                             <  2:254633/139‑1 (MQ=255)                                                        caTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                      <  2:301915/139‑1 (MQ=255)                                                             cTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                             <  2:84754/111‑1 (MQ=255)                                                             cTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                             >  1:84754/1‑111 (MQ=255)                                                             cTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                 <  1:705083/139‑1 (MQ=255)                                                                  gggCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                            <  1:264220/139‑1 (MQ=255)                                                                   ggcggcGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCCCTCGGCATTTATGGTGCTGGtt                                                           >  1:461521/1‑139 (MQ=255)                                                                     cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAt                                                         <  2:111522/139‑1 (MQ=255)                                                                                  atcatcAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                            <  1:545791/139‑1 (MQ=255)                                                                                        aGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGtt                                      <  1:339442/139‑1 (MQ=255)                                                                                                 aCGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTTAATTTGCCGAGTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                             <  2:540240/139‑1 (MQ=255)                                                                                                       ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                       <  1:204950/139‑1 (MQ=255)                                                                                                       ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                       <  1:396416/139‑1 (MQ=255)                                                                                                        tACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                      <  2:461521/139‑1 (MQ=255)                                                                                                        tACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                      <  2:789190/139‑1 (MQ=255)                                                                                                            tGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                      >  1:447730/1‑135 (MQ=255)                                                                                                            tGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                      <  2:447730/135‑1 (MQ=255)                                                                                                            tGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGAc                  >  2:626836/1‑139 (MQ=255)                                                                                                            tGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGAc                  <  1:626836/139‑1 (MQ=255)                                                                                                                 gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtat              >  1:680439/1‑139 (MQ=255)                                                                                                                 gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                 <  1:489168/119‑1 (MQ=255)                                                                                                                 gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                 >  2:489168/1‑119 (MQ=255)                                                                                                                 gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata             >  1:473618/1‑139 (MQ=255)                                                                                                                 gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata             >  1:128240/1‑139 (MQ=255)                                                                                                                 gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata             >  2:359083/1‑139 (MQ=255)                                                                                                                 gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata             >  1:609333/1‑139 (MQ=255)                                                                                                                  tgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat            <  2:209802/139‑1 (MQ=255)                                                                                                                       cTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGcgcg       <  2:128240/139‑1 (MQ=255)                                                                                                                       cTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGcgcg       <  1:359083/139‑1 (MQ=255)                                                                                                                         ttAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGaa     <  2:473618/139‑1 (MQ=255)                                                                                                                            gTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc  <  1:759033/139‑1 (MQ=255)                                                                                                                            gTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc  >  2:640355/1‑139 (MQ=255)                                                                                                                            gTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc  <  1:640355/139‑1 (MQ=255)                                                                                                                                  cGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                            <  1:614154/75‑1 (MQ=255)                                                                                                                                  cGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                            >  2:614154/1‑75 (MQ=255)                                                                                                                                         |                                                                                                                               GCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAC  >  NC_000913/2601875‑2602135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑