Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,763,869 (T)7→8 coding (1285/1617 nt) yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,763,8621.T100.0% 91.5 / NA 27I431N (ATC→AAC) yfjLCP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (16/11);  total (16/11)

ATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG  >  NC_000913/2763735‑2763995
                                                                                                                                |                                                                                                                                     
aTTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttttttgcg                                                                                                                            <  2:442147/139‑5 (MQ=255)
       tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTa                                                                                                                      >  1:35172/1‑139 (MQ=255)
          tAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCg                                                                                                                   >  2:495588/1‑139 (MQ=255)
               cACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCa                                                                                                              >  2:37179/1‑139 (MQ=255)
                 cGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAAt                                                                                                            >  2:31543/1‑139 (MQ=255)
                        aacaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGAAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAAt                                                                                                     >  2:224074/1‑139 (MQ=255)
                           aaaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTc                                                                                                  <  2:35172/139‑1 (MQ=255)
                            aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc                                                                                                 <  1:31543/139‑1 (MQ=255)
                                tGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGt                                                                                             <  1:37179/139‑1 (MQ=255)
                                      gATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACt                                                                                       <  2:637641/139‑1 (MQ=255)
                                       aTCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTg                                                                                      >  2:574040/1‑139 (MQ=255)
                                          gAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAACTGTCCTCGTACAACTGATc                                                                                   >  2:344056/1‑139 (MQ=255)
                                                aGCATCGAAAGGTTGGCTACCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttttt                                                                                                                                >  2:765338/1‑87 (MQ=255)
                                                aGCATCGAAAGGTTGGCTACCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAttttttt                                                                                                                                <  1:765338/87‑1 (MQ=255)
                                                            ttGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCt                                                                 >  2:682323/1‑139 (MQ=255)
                                                                   ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa                                                          <  1:43330/139‑1 (MQ=255)
                                                                                                aaGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGaa                             >  2:766192/1‑139 (MQ=255)
                                                                                                  gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAAc                                     <  2:338136/129‑1 (MQ=255)
                                                                                                  gCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAAc                                     >  1:338136/1‑129 (MQ=255)
                                                                                                      gTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTgcgcg                                                                                                                           >  2:229144/1‑39 (MQ=255)
                                                                                                      gTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTgcgcg                                                                                                                           <  1:229144/39‑1 (MQ=255)
                                                                                                            aTCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAg                 <  2:241903/139‑1 (MQ=255)
                                                                                                               gTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGcctgtctcttatac              <  1:766192/139‑13 (MQ=255)
                                                                                                                tCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa                                                          <  2:801204/94‑1 (MQ=255)
                                                                                                                tCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa                                                          >  1:801204/1‑94 (MQ=255)
                                                                                                                tCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa             >  2:356997/1‑139 (MQ=255)
                                                                                                                    tAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCAc         <  1:682323/139‑1 (MQ=255)
                                                                                                                    tAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCAc         >  1:28224/1‑139 (MQ=255)
                                                                                                                          cGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTg   >  2:316003/1‑139 (MQ=255)
                                                                                                                           gAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTgg  >  1:484603/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                     
ATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG  >  NC_000913/2763735‑2763995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: