Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 371,588 (G)6→7 coding (350/867 nt) mhpC → 2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913371,5821.G100.0% 61.0 / NA 17M115R (ATG→AGG) mhpC2‑hydroxy‑6‑ketonona‑2,4‑dienedioic acid hydrolase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (10/7);  total (10/7)

CTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAAT  >  NC_000913/371446‑371715
                                                                                                                                         |                                                                                                                                     
ctgctgGAATGTCCGGGTTGGGGCCAGAGATATTCGGTCTTGAATAGAGGTTCGCCATCGGATCTGAATGCACTAATCCTGAAAAGCGTGGTAGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATgg                                                                                                                                     <  2:593349/139‑1 (MQ=255)
           tCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAg                                                                                                                           >  2:105639/1‑139 (MQ=255)
                gTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCtg                                                                                                                      >  2:120526/1‑139 (MQ=255)
                       cAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGtt                                                                                                               <  1:105639/139‑1 (MQ=255)
                                ttCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTaa                                                                                                      <  1:120526/139‑1 (MQ=255)
                                               tGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGt                                                                                       >  1:665841/1‑139 (MQ=255)
                                                                       aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcgg                                                               >  1:231200/1‑139 (MQ=255)
                                                                       aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGggcgg                                                               >  1:219276/1‑139 (MQ=255)
                                                                       aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATg                                                                                                                                      >  1:379993/1‑67 (MQ=255)
                                                                       aCGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATg                                                                                                                                      <  2:379993/67‑1 (MQ=255)
                                                                             ccTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTAc                                                         >  1:606969/1‑139 (MQ=255)
                                                                                gAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACggg                                                      <  2:606969/139‑1 (MQ=255)
                                                                                      cGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCAt                                                >  1:8005/1‑139 (MQ=255)
                                                                                                     ggATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGcc                                 <  2:219276/139‑1 (MQ=255)
                                                                                                           cGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttgtt                                       <  1:48711/127‑1 (MQ=255)
                                                                                                           cGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAgtttgtt                                       >  2:48711/1‑127 (MQ=255)
                                                                                                                aaaTCCACCTGCTGGGCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCg                      <  2:665841/139‑1 (MQ=255)
                                                                                                                              ggCAACTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGa        >  2:93467/1‑139 (MQ=255)
                                                                                                                                   cTCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGaa   >  1:99661/1‑139 (MQ=255)
                                                                                                                                    tCGATGGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAAt  <  2:231200/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CTGCTGGATTGTCCGGGTTGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATCCTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATGGGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTGCTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATTAAGCGACTGAAT  >  NC_000913/371446‑371715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: