| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 751,737 | (G)6→7 | coding (433/2448 nt) | ybgQ ← | putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 751,731 | 1 | . | G | 95.0% | 66.7 / ‑2.7 | 20 | coding (439/2448 nt) | ybgQ | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (7/12); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
GCGCCATGCCCCGGCGTTAATTCCGGCGGTACCGTAGGCATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCT > NC_000913/751594‑751862 | gcgcCATGCCCCGGCGTTAATTCCGGCGGTACCGTAGGCATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTaga‑g > 1:170046/1‑139 (MQ=255)gcgcCATGCCCCGGCGTTAATTCCGGCGGTACCGTAGGCATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTaga‑g < 2:369691/139‑1 (MQ=255) tACCGTAGGCATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACg < 1:747592/139‑1 (MQ=255) gTAGGCATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑GGGGGGAGCCCAGTTTTCTGAGTGCCACgccagc < 2:170046/139‑1 (MQ=255) aTTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGc < 2:255156/139‑1 (MQ=255) tAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGaa < 1:21715/139‑1 (MQ=255) tAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGaa < 1:154558/139‑1 (MQ=255) tAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGaa < 1:392238/139‑1 (MQ=255) tAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑gggggggagc > 1:434987/1‑97 (MQ=255) tAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCGGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑gggggggagc < 2:434987/101‑5 (MQ=255) cGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTg > 2:216259/1‑108 (MQ=255) cGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTg < 1:216259/108‑1 (MQ=255) ggTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCtt < 1:737596/139‑1 (MQ=255) cAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGa > 2:651894/1‑139 (MQ=255) aTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGaa < 1:259358/87‑1 (MQ=255) aTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGaa > 2:259358/1‑87 (MQ=255) ccGGCAACACCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGaaa > 1:245133/1‑139 (MQ=255) cacCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTc < 1:678355/116‑1 (MQ=255) cacCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTc > 2:678355/1‑116 (MQ=255) cacCTTCTTTCCATGTAGAGGGGGGGAGCCCAGTTTTCGGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATac < 2:251835/139‑1 (MQ=255) ttcttTCCATGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCg > 1:24280/1‑139 (MQ=255) aTGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGatctatct < 2:24280/139‑1 (MQ=255) tGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGa < 2:212777/87‑1 (MQ=255) tGTAGAGGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGa > 1:212777/1‑87 (MQ=255) | GCGCCATGCCCCGGCGTTAATTCCGGCGGTACCGTAGGCATTCAGGTTAGTGCTGCTGCTGCCGTCCTGTGGGCGGTAGCTGCTGGCAAACAAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGA‑GGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCT > NC_000913/751594‑751862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |