Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,491,119	IS5 (+) +4 bp	coding (218‑221/531 nt)	cybB →	cytochrome b561

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1491119 =	0 (0.000)	51 (1.390)	27/224	0.3	100%	coding (218/531 nt)	cybB	cytochrome b561
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1491122	0 (0.000)	36 (0.980)	24/224	0.4	100%	coding (221/531 nt)	cybB	cytochrome b561
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1490986‑1491122 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGAT  <  NC_000913/2290113‑2289978                                                                                                                                                                                                                                                                                   TTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGG                                                                                                                                        <  2:394131/139‑1           TCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAA                                                                                                                              <  2:539524/139‑1                 CACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTC                                                                                                                        >  2:782693/1‑139                  ACGTAGCGATGGGCCACTCATCAATGTGGTTCCTGTTTCCTGTGGCATCTCAATTCTTGTGCTGATGGTCTTTCGTCTTTTGTTAAGGCTGAAATACCCAACCTCTCCGATTATACCTAAGGAAGGTGCTAACAAGTCC                                                                                                                       <  2:86967/139‑1                  ACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTCTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCC                                                                                                                       <  2:628223/139‑1                    GTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCT                                                                                                                     <  1:713917/139‑1                                       CAACATGATTCATGTTTCCTGAGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGT                                                                                                  <  1:657833/139‑1                                               TTCATGTTTCCTGTGTCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGA                                                                                          >  2:431202/1‑139                                               TTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGA                                                                                          >  1:350870/1‑139                                               TTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAG                                                                                                                          >  1:168689/1‑107                                               TTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAG                                                                                                                          <  2:168689/107‑1                                                  ATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAATGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCC                                                                                       <  2:530631/139‑1                                                   TGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                      <  1:168485/139‑1                                                          TGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACA                                                                               >  2:777145/1‑139                                                                 TCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAG                                                                              <  1:84440/133‑1                                                                 TCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAG                                                                              >  2:84440/1‑133                                                                   TCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCC                                                                                                                       <  1:578073/90‑1                                                                   TCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCC                                                                                                                       >  2:578073/1‑90                                                                            GTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCC                                                                                                                      >  2:187386/1‑82                                                                            GTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCC                                                                                                                      <  1:187386/82‑1                                                                            GTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAAC                                                                                                                             <  2:235762/75‑1                                                                            GTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAAC                                                                                                                             >  1:235762/1‑75                                                                                      TCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCT                                                   >  2:698880/1‑139                                                                                          TCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGC                                               <  2:350870/139‑1                                                                                                 TTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATA                                                                                                                 >  1:429662/1‑66                                                                                                 TTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATA                                                                                                                 <  2:429662/66‑1                                                                                                                TACCCAACCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAG                         <  2:732579/139‑1                                                                                                                       CCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                      >  1:276698/1‑71                                                                                                                       CCCCGCCGATTATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                      <  2:276698/71‑1                                                                                                                                 TATACCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAA        >  2:516456/1‑139                                                                                                                                     CCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAG    >  2:235593/1‑139                                                                                                                                      CTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA   <  1:520157/139‑1                                                                                                                                      CTAAGAAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGA   <  2:257628/139‑1                                                                                                                                       TAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGAT  >  2:325043/1‑139                                                                                                                                         AGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                      >  2:80377/1‑53                                                                                                                                         AGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCA                                                                                      <  1:80377/53‑1                                                                                                                                          GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGG                                                                                           <  1:65324/47‑1                                                                                                                                          GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGG                                                                                           >  2:65324/1‑47                                                                                                                                                                                                                                                                                   TTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1490986‑1491122 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGAT  <  NC_000913/2290113‑2289978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.