Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 60,432 G→A Q945* (CAG→TAG)  rapA ← RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091360,4320GA100.0% 71.5 / NA 22Q945* (CAG→TAG) rapARNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

GATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGACAGTTTTTCGTCGGCTTCGTTACGCGCTGCATCAATCAATGCACG  >  NC_000913/60299‑60567
                                                                                                                                     |                                                                                                                                       
gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGa                                                                                                                                     >  2:582208/1‑138 (MQ=255)
gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGa                                                                                                                                     <  1:582208/138‑1 (MQ=255)
gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGAc                                                                                                                                    >  1:342260/1‑139 (MQ=255)
gATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGAc                                                                                                                                    >  2:516704/1‑139 (MQ=255)
        accaGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGctc                                                                                                                            >  2:492746/1‑139 (MQ=255)
          caGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCtctc                                                                                                                          >  2:282829/1‑139 (MQ=255)
          caGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCAGCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCtctc                                                                                                                          >  1:429020/1‑139 (MQ=255)
                   tGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGt                                                                                                                 >  1:771027/1‑139 (MQ=255)
                           aTTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTtcgt                                                                                                         <  2:347305/139‑1 (MQ=255)
                                        aTCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGtt                                                                                            <  2:396038/139‑1 (MQ=255)
                                             cATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGt                                                                                       <  1:674162/139‑1 (MQ=255)
                                              aTTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGtt                                                                                      <  2:429020/139‑1 (MQ=255)
                                               ttttCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTc                                                                                     <  1:563476/139‑1 (MQ=255)
                                               ttttCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTc                                                                                     <  2:342260/139‑1 (MQ=255)
                                                                                      cAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGAc                                              <  2:771027/139‑1 (MQ=255)
                                                                                                         cctgcctgATCCAGGCTTTCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGACAGTTTTTCGTCGGCTTCGt                           >  1:203307/1‑139 (MQ=255)
                                                                                                                          ttCCATTACCGACTGACGGTGGCTCTGAATAGCGGGCAGTTCGTCGTCAAGAATGTTCGGGTTCACTGCACGAAGAGCTTCCAGACGAGACGGCTCGGCAGACAGTTTTTCGTCGGCTTCGTTACGCGCTGCatcaatc          <  2:203307/139‑1 (MQ=255)
                                                                                                                           tCCATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGACAGTTTTTCGTCGGCTTCGTTACGCGCTGCatcaatca         >  1:105906/1‑139 (MQ=255)
                                                                                                                            ccATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCa                                                                           <  2:204925/72‑1 (MQ=255)
                                                                                                                            ccATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCa                                                                           >  1:204925/1‑72 (MQ=255)
                                                                                                                            ccATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGACAGTTTTTCGTCGGCTTCGTTa                         <  1:704753/122‑1 (MQ=255)
                                                                                                                            ccATTACCTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGACAGTTTTTCGTCGGCTTCGTTa                         >  2:704753/1‑122 (MQ=255)
                                                                                                                                  ccTACTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGACAGTTTTTCGTCGGCTTCGTTACGCGCTGCATCGATCAATGCACg  >  1:524073/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GATAACCAACCAGGGTTCCTGCGGTGGATTGTAGTTTTCCATCCCCATTTTCGGCTCCGTTACTGATGCGTTACAACGATCAAACGCAGGGCATCCAGACGCCAACCTGCCTGATCCAGGCTTTCCATTACCTGCTGACGGTTGCTCTCAATGGCGGTCAGTTCGTCGTCACGAATGTTCGGGTTCACTGCACGCAGAGCTTCCAGACGAGACAGCTCGGCAGACAGTTTTTCGTCGGCTTCGTTACGCGCTGCATCAATCAATGCACG  >  NC_000913/60299‑60567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: