Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 917,894 A→G E474E (GAA→GAG ybjD → putative OLD family ATP‑dependent endonuclease, DUF2813 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913917,8940AG100.0% 95.3 / NA 26E474E (GAA→GAGybjDputative OLD family ATP‑dependent endonuclease, DUF2813 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/9);  total (17/9)

GGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATGAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCA  >  NC_000913/917780‑918024
                                                                                                                  |                                                                                                                                  
ggTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAg                                                                                                                  >  2:5271/1‑133 (MQ=255)
ggTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAg                                                                                                                  <  1:5271/133‑1 (MQ=255)
 gTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGAtttt                                                                                                           >  2:721072/1‑139 (MQ=255)
      tGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGAt                                                                                                      >  1:280010/1‑139 (MQ=255)
      tGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGAt                                                                                                      >  1:432738/1‑139 (MQ=255)
                  aGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAggg                                                                                                                <  1:170995/117‑1 (MQ=255)
                  aGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAggg                                                                                                                >  2:170995/1‑117 (MQ=255)
                              tGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATc                                                                              >  2:619109/1‑139 (MQ=255)
                              tGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATc                                                                              >  2:439666/1‑139 (MQ=255)
                                ccGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATccc                                                                            <  1:619109/139‑1 (MQ=255)
                                      cGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGtt                                                                                                 >  2:736654/1‑112 (MQ=255)
                                      cGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGtt                                                                                                 <  1:736654/112‑1 (MQ=255)
                                         tACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCaa                                                                                 >  1:728920/1‑125 (MQ=255)
                                         tACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCaa                                                                                 <  2:728920/125‑1 (MQ=255)
                                                ccTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATg                                                            >  2:396311/1‑139 (MQ=255)
                                                ccTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATg                                                            >  1:227355/1‑139 (MQ=255)
                                                                   gAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATGAATCTACGCAAAATTAtct                                         <  1:323019/139‑1 (MQ=255)
                                                                      gCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATccc                                                                            >  2:108670/1‑101 (MQ=255)
                                                                      gCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATccc                                                                            <  1:108670/101‑1 (MQ=255)
                                                                                              ttACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCa                                                                                              <  1:7125/59‑1 (MQ=255)
                                                                                              ttACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCa                                                                                              >  2:7125/1‑59 (MQ=255)
                                                                                              ttACCGGCGCTGGATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATGAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAAc              >  2:648355/1‑139 (MQ=255)
                                                                                                         ggATATGGAGCATTTTATGTATCGCCAGGGATTTTCCg                                                                                                        >  2:510121/1‑38 (MQ=255)
                                                                                                         ggATATGGAGCATTTTATGTATCGCCAGGGATTTTCCg                                                                                                        <  1:510121/38‑1 (MQ=255)
                                                                                                          gATATGGAGCATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATGAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATTTTGCCa  >  1:544778/1‑139 (MQ=255)
                                                                                                               ggAGCATTTTATGTATCGCCAGGGATTTTCCGAtgtg                                                                                                   >  1:560296/1‑37 (MQ=255)
                                                                                                                  |                                                                                                                                  
GGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATGAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCA  >  NC_000913/917780‑918024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: