Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,385,656 A→G E49G (GAG→GGG)  ycjF → UPF0283 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,385,6560AG100.0% 71.3 / NA 21E49G (GAG→GGG) ycjFUPF0283 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/10);  total (11/10)

CACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGG  >  NC_000913/1385530‑1385777
                                                                                                                              |                                                                                                                         
cACGTATTGATTTCGACAGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGa                                                                                                               <  1:417328/139‑1 (MQ=255)
                             gTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGa                                                                                  >  2:601477/1‑139 (MQ=255)
                             gTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGa                                                                                  >  2:148513/1‑139 (MQ=255)
                             gTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGAAGCGTTACGTCCGa                                                                                  >  2:199763/1‑139 (MQ=255)
                             gTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGACAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGa                                                                                  >  1:627415/1‑139 (MQ=255)
                               cGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGaaa                                                                                >  2:446270/1‑139 (MQ=255)
                                        tCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCtg                                                                       >  2:225025/1‑139 (MQ=255)
                                               ttCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCa                                                                <  1:351761/139‑1 (MQ=255)
                                                tCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGc                                                                                                                      >  2:679104/1‑84 (MQ=255)
                                                tCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGc                                                                                                                      <  1:679104/84‑1 (MQ=255)
                                                   gggCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAAt                                                            >  2:512260/1‑139 (MQ=255)
                                                        cagcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGa                                                       >  1:656026/1‑139 (MQ=255)
                                                         agcagACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCCCAGGAGGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGACCGAAACGAAGCCTGTGGCGCAAAATGGTGaa                                                      >  2:602341/1‑138 (MQ=255)
                                                                 tttGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGTCTGTGGCGCAAAATGGTGATGGGAGggc                                              <  1:498512/139‑1 (MQ=255)
                                                                      cGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGggctggct                                         <  1:148513/139‑1 (MQ=255)
                                                                                                    cACGCTCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGgt           <  2:377856/139‑1 (MQ=255)
                                                                                                         tCGACGAAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACa      <  1:225025/139‑1 (MQ=255)
                                                                                                            acgaAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGa                                                                                                   <  2:147361/43‑1 (MQ=255)
                                                                                                            acgaAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGa                                                                                                   >  1:147361/1‑43 (MQ=255)
                                                                                                             cgaAGCGCAGGAAGAAGGGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTgg  <  2:583211/139‑1 (MQ=255)
                                                                                                             cgaAGCGCAGGAAGAAGGGGGACAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTgg  <  2:627415/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                         
CACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAATTCAGGGCGCAGCAGACCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGG  >  NC_000913/1385530‑1385777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑