Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,581,323	G→A	T401I (ACA→ATA)	ydeN ←	putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,581,323	0	G	A	100.0%	74.5 / NA	22	T401I (ACA→ATA)	ydeN	putative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/11);  total (11/11)

TATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTGTCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTAT  >  NC_000913/1581205‑1581437                                                                                                                       |                                                                                                                   tATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCg                                                                                                >  2:68080/1‑139 (MQ=255) tATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCg                                                                                                >  1:617564/1‑139 (MQ=255)           aGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCt                                                                                      >  1:350585/1‑139 (MQ=255)             aTTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTg                                                                                    <  1:68080/139‑1 (MQ=255)                       ggCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       >  1:241023/1‑126 (MQ=255)                       ggCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       <  2:241023/126‑1 (MQ=255)                       ggCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTAc                                                                          >  1:661971/1‑139 (MQ=255)                                        aTCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTtttcct                                                         >  2:638627/1‑139 (MQ=255)                                             gCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTcca                                                    <  1:638627/139‑1 (MQ=255)                                              cAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTccac                                                   <  2:617564/139‑1 (MQ=255)                                               aaCCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTccacc                                                  >  1:552489/1‑139 (MQ=255)                                                         gcaAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACAt                                        >  1:389849/1‑139 (MQ=255)                                                          caAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACAtt                                       <  2:350585/139‑1 (MQ=255)                                                          caAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACAtt                                       <  2:424577/139‑1 (MQ=255)                                                                 aCGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGgtgt                                <  1:224791/139‑1 (MQ=255)                                                                     cATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGt                            <  2:389849/139‑1 (MQ=255)                                                                     cATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGt                            <  2:552489/139‑1 (MQ=255)                                                                               aaGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGa                  <  2:661971/139‑1 (MQ=255)                                                                                 ggTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       >  2:195894/1‑68 (MQ=255)                                                                                 ggTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       <  1:195894/68‑1 (MQ=255)                                                                                             tGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCtt    >  1:272458/1‑139 (MQ=255)                                                                                               cTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTtat  >  1:131639/1‑139 (MQ=255)                                                                                                                       |                                                                                                                   TATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTGTCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTAT  >  NC_000913/1581205‑1581437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑