Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,621,054	A→G	H28H (CAT→CAC)	eamA ←	cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,621,054	0	A	G	100.0%	61.6 / NA	17	H28H (CAT→CAC)	eamA	cysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/8);  total (9/8)

TGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAA  >  NC_000913/1620923‑1621185                                                                                                                                    |                                                                                                                                    tGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGcc                                                                                                                              <  1:419261/139‑1 (MQ=255)                          aCCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAg                                                                                                     >  1:49218/1‑139 (MQ=255)                                    caAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACg                                                                                           <  2:49218/139‑1 (MQ=255)                                       aTTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGAcc                                                                                        >  1:67311/1‑139 (MQ=255)                                                        gTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCaa                                                                       <  2:67311/139‑1 (MQ=255)                                                              cGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAcccc                                                                 >  1:699929/1‑139 (MQ=255)                                                                           aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGcc                                                                                                                              >  1:120065/1‑65 (MQ=255)                                                                           aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGcc                                                                                                                              <  2:120065/65‑1 (MQ=255)                                                                               ggAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTc                                                >  2:231121/1‑139 (MQ=255)                                                                                      cGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGcc                                         >  2:88820/1‑139 (MQ=255)                                                                                        aCCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctc                                       <  1:458832/139‑1 (MQ=255)                                                                                           aGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGa                                    >  1:370335/1‑139 (MQ=255)                                                                                                  aGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCAt                             <  2:699929/139‑1 (MQ=255)                                                                                                       aaaCCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctct                                      >  1:499060/1‑125 (MQ=255)                                                                                                       aaaCCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCctct                                      <  2:499060/125‑1 (MQ=255)                                                                                                       aaaCCGGCCAGCATCAGCGGTGGCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtttt                        >  1:633595/1‑139 (MQ=255)                                                                                                                        cgttcgCATGTTGTCAAGCCCCACTTTGATGACCACAAAATTCAGCCCCCATACCACCACTACCATTAGCGCCAACCCCCCATCTTTCCCCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAg       <  2:230049/134‑1 (MQ=255)                                                                                                                             gCATGTTGTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGaa  <  2:417646/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    TGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAA  >  NC_000913/1620923‑1621185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑