| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,756,464 | T→C | V256A (GTT→GCT) | pykF → | pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,756,464 | 0 | T | C | 100.0% | 85.0 / NA | 24 | V256A (GTT→GCT) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/11); total (13/11) | |||||||||||
CGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG > NC_000913/1756333‑1756598 | cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGTTGTAGAAATCCCGGTAGAAGAAGCTAt < 2:481441/135‑1 (MQ=255)cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTACGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTAt > 1:481441/1‑135 (MQ=255) cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGACGCTATCTTCGCCCag < 2:538813/139‑1 (MQ=255) cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCag < 1:157007/139‑1 (MQ=255) gAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCAc > 2:797385/1‑139 (MQ=255) ccTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa < 1:797385/139‑1 (MQ=255) ccTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa > 2:199726/1‑139 (MQ=255) cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACt > 2:106078/1‑139 (MQ=255) gacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg < 2:10994/139‑1 (MQ=255) ccTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAg < 1:199726/139‑1 (MQ=255) catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGccc > 1:103348/1‑62 (MQ=255) catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGccc < 2:103348/62‑1 (MQ=255) gTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAcc < 2:177600/139‑1 (MQ=255) gcgcGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc > 1:444389/1‑51 (MQ=255) gcgcGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc < 2:444389/51‑1 (MQ=255) cgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCg > 2:426207/1‑64 (MQ=255) cgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCg < 1:426207/64‑1 (MQ=255) tGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg > 2:742991/1‑139 (MQ=255) cGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt < 2:777274/139‑1 (MQ=255) tCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa > 2:585957/1‑139 (MQ=255) tCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa > 2:640447/1‑139 (MQ=255) ccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg > 2:663616/1‑139 (MQ=255) aagaagCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa > 1:441676/1‑139 (MQ=255) gaagCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg > 1:77122/1‑139 (MQ=255) | CGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG > NC_000913/1756333‑1756598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |