Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,281	T→C	E532E (GAA→GAG)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,281	0	T	C	100.0%	168.5 / NA	45	E532E (GAA→GAG)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/19);  total (26/19)

TGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGATAATG  >  NC_000913/2318149‑2318416                                                                                                                                     |                                                                                                                                        tGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATc                                                                                                                                   >  1:518525/1‑139 (MQ=255)         ccGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGaa                                                                                                                           <  2:141519/139‑1 (MQ=255)         ccGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGaa                                                                                                                           <  2:518525/139‑1 (MQ=255)             ttCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCgag                                                                                                                             >  2:794656/1‑133 (MQ=255)             ttCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCgag                                                                                                                             <  1:794656/133‑1 (MQ=255)                          tACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGa                                                                                                          <  2:452511/139‑1 (MQ=255)                                    aCTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaa                                                                                                >  1:396113/1‑139 (MQ=255)                                       gCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                                                                             <  2:788147/139‑1 (MQ=255)                                       gCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                                                                             <  2:396113/139‑1 (MQ=255)                                         ttGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                                                                             <  2:792396/137‑1 (MQ=255)                                         ttGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTcaacaa                                                                                             >  1:792396/1‑137 (MQ=255)                                          tGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAg                                                                                          >  2:644050/1‑139 (MQ=255)                                                    caGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAagag                                                                                <  2:746440/139‑1 (MQ=255)                                                       cGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAgttg                                                                             >  2:791635/1‑139 (MQ=255)                                                                  tGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGt                                                                  >  1:305684/1‑139 (MQ=255)                                                                    gCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCa                                                                >  1:486967/1‑139 (MQ=255)                                                                     cGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCAc                                                               <  1:644050/139‑1 (MQ=255)                                                                     cGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCAc                                                               <  2:204443/139‑1 (MQ=255)                                                                        tGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAg                                                            >  2:690708/1‑139 (MQ=255)                                                                         gATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGc                                                           >  2:516007/1‑139 (MQ=255)                                                                                    tcaCTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCAcc                                                              <  2:510983/125‑1 (MQ=255)                                                                                    tcaCTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCAcc                                                              >  1:510983/1‑125 (MQ=255)                                                                                     caCTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCt                                               >  1:166086/1‑139 (MQ=255)                                                                                         tCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCg                                           >  1:637538/1‑139 (MQ=255)                                                                                                gTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAacaaca                                                                                           >  2:181866/1‑84 (MQ=255)                                                                                                gTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAacaaca                                                                                           <  1:181866/84‑1 (MQ=255)                                                                                                gTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAActc                                    >  1:221441/1‑139 (MQ=255)                                                                                                 tGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGt                                         >  2:212432/1‑133 (MQ=255)                                                                                                 tGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGt                                         <  1:212432/133‑1 (MQ=255)                                                                                                           cgcgGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCtt                                  >  1:676776/1‑130 (MQ=255)                                                                                                           cgcgGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCtt                                  <  2:676776/130‑1 (MQ=255)                                                                                                           cgcgGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCa                         >  1:485893/1‑139 (MQ=255)                                                                                                           cgcgGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCa                         >  2:561746/1‑139 (MQ=255)                                                                                                            gcgGTTCGATCTTCAACTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCaa                        <  2:12749/139‑1 (MQ=255)                                                                                                                          aaCTGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTAc          >  2:265235/1‑139 (MQ=255)                                                                                                                             tGTTCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGa       <  2:330022/139‑1 (MQ=255)                                                                                                                               ttCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCttt                                             <  2:330503/99‑1 (MQ=255)                                                                                                                               ttCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCttt                                             >  1:330503/1‑99 (MQ=255)                                                                                                                               ttCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAGCTCTTTGGTTTGCAACAGATCCAGCTTACCGATa     >  1:516680/1‑139 (MQ=255)                                                                                                                               ttCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGa       >  1:286298/1‑137 (MQ=255)                                                                                                                               ttCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGa       <  2:286298/137‑1 (MQ=255)                                                                                                                               ttCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGATa     >  1:325913/1‑139 (MQ=255)                                                                                                                               ttCCGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGATa     >  1:121411/1‑139 (MQ=255)                                                                                                                                 ccGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGATAAt   >  1:604843/1‑139 (MQ=255)                                                                                                                                  cGACTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGATAATg  <  1:561746/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAACAGGCTGGAAGAGTTGTTCATTGCCGTCACCAGCCGATCGACGCCTTTCGGTAACTCTTTGGTTTGCAACAGATCCAGGTTACCGATAATG  >  NC_000913/2318149‑2318416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑