Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,319,725 | T→C | E51G (GAA→GGA) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,319,725 | 0 | T | C | 100.0% | 156.8 / NA | 42 | E51G (GAA→GGA) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/21); total (21/21) |
TTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAG > NC_000913/2319594‑2319860 | tttCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACg < 2:602241/139‑1 (MQ=255) cACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGa > 1:701075/1‑139 (MQ=255) cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGAtt < 1:160071/139‑1 (MQ=255) cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGAtt < 1:731522/139‑1 (MQ=255) cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGAtt < 1:30028/139‑1 (MQ=255) ggggAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATtctc < 1:436868/139‑1 (MQ=255) cacaCCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGt < 1:157335/139‑1 (MQ=255) ccGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACg > 2:431532/1‑139 (MQ=255) gTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCg > 2:614328/1‑126 (MQ=255) gTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCg < 1:614328/126‑1 (MQ=255) tCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTaa > 2:12186/1‑139 (MQ=255) aTAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTa > 1:766726/1‑139 (MQ=255) ttCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGAt > 2:439052/1‑139 (MQ=255) gCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGaaa < 1:431532/139‑1 (MQ=255) ctTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCa < 1:366423/139‑1 (MQ=255) tttCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCag < 2:390855/139‑1 (MQ=255) ttCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCaga > 1:762802/1‑139 (MQ=255) atcaGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAAc > 2:710678/1‑139 (MQ=255) gagGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCa < 2:701075/139‑1 (MQ=255) gTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCGCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTGACGCCAATg > 2:548888/1‑139 (MQ=255) tGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc < 2:231780/139‑1 (MQ=255) cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc > 2:513271/1‑139 (MQ=255) cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc > 2:462560/1‑139 (MQ=255) cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc > 2:434183/1‑139 (MQ=255) cGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGctc < 1:439052/139‑1 (MQ=255) ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACg > 2:745740/1‑82 (MQ=255) ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACg < 1:745740/82‑1 (MQ=255) ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTg > 1:231486/1‑139 (MQ=255) ttGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTg < 1:462560/139‑1 (MQ=255) aGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc > 2:297844/1‑127 (MQ=255) aGCGCTGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGc < 1:297844/127‑1 (MQ=255) tGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc > 2:69611/1‑136 (MQ=255) tGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAgc < 1:69611/136‑1 (MQ=255) tGAGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGcgcg > 1:801902/1‑139 (MQ=255) aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg > 1:232604/1‑139 (MQ=255) aGCCTGATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAg < 2:766726/139‑1 (MQ=255) gATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATg > 2:199598/1‑114 (MQ=255) gATCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATg < 1:199598/114‑1 (MQ=255) tCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGc < 2:108254/42‑1 (MQ=255) tCGGAACTCAGATTAAATCCCTGACGAATTTCCGATTCTCGc > 1:108254/1‑42 (MQ=255) aaaTCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGaaaa > 1:214958/1‑139 (MQ=255) aaTCCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAaag < 1:727749/139‑1 (MQ=255) | TTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTAACGCATTAACGATGTAAAAAACGGATGAAAAAGCAATCAACAGCCAGAGCACTAACGCCAATGCTCTGAACATGTAGCGCGAGGCTTTCAGGGTTGTACGAAAAG > NC_000913/2319594‑2319860 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |