Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,657,358	G→A	A526A (GCC→GCT)	hscA ←	DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,657,358	0	G	A	100.0%	99.9 / NA	29	A526A (GCC→GCT)	hscA	DnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/10);  total (19/10)

CAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGC  >  NC_000913/2657240‑2657491                                                                                                                       |                                                                                                                                      cagcaTCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGc                                                                                                                   >  1:637795/1‑139 (MQ=255)   gcaTCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCAt                                                                                                                 >  1:208324/1‑139 (MQ=255)           aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt                                                                                                         <  2:785831/139‑1 (MQ=255)           aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt                                                                                                         <  2:208324/139‑1 (MQ=255)           aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt                                                                                                         >  1:71876/1‑139 (MQ=255)           aaTGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCAtt                                                                                                         <  1:338317/139‑1 (MQ=255)                   gACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCttt                                                                                                 >  1:545111/1‑139 (MQ=255)                              gcgcTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAg                                                                                      >  1:608622/1‑139 (MQ=255)                               cgcTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGc                                                                                     >  1:594894/1‑139 (MQ=255)                                           cgGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATc                                                                         >  2:138014/1‑139 (MQ=255)                                           cgGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATc                                                                         >  2:217042/1‑139 (MQ=255)                                              cagcagcagCCGGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACCTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGt                                                                      >  2:467092/4‑139 (MQ=255)                                                tCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCa                                                                    <  1:408884/139‑1 (MQ=255)                                                  agcagcCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGa                                                                  <  2:637795/139‑1 (MQ=255)                                                           cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCAt                                                                                                                 <  1:326153/83‑1 (MQ=255)                                                           cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCAt                                                                                                                 >  2:326153/1‑83 (MQ=255)                                                           cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGAc                                                         >  2:675403/1‑139 (MQ=255)                                                                cGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                    <  1:138014/139‑1 (MQ=255)                                                                  tGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGa                                                  >  2:705573/1‑139 (MQ=255)                                                                            ccAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa                                        >  1:342275/1‑139 (MQ=255)                                                                            ccAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa                                        <  2:594894/139‑1 (MQ=255)                                                                            ccAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa                                        >  1:742792/1‑139 (MQ=255)                                                                              aGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACg                                      >  1:224644/1‑139 (MQ=255)                                                                                 acacGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCt                                   >  1:682962/1‑139 (MQ=255)                                                                                     gcgcCGCTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAAc                               >  2:540178/1‑139 (MQ=255)                                                                                       gccgcTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGc                             <  1:540178/139‑1 (MQ=255)                                                                                          gcTTCTACTTTTTGTTCTGCCAGCATTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCgg                          <  2:682962/139‑1 (MQ=255)                                                                                                                  aTTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGc  >  2:358047/1‑139 (MQ=255)                                                                                                                  aTTCGAGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGc  >  1:338331/1‑139 (MQ=255)                                                                                                                       |                                                                                                                                      CAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGC  >  NC_000913/2657240‑2657491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑