Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,786,768	C→T	pseudogene (372/2253 nt)	ygaQ →	uncharacterized protein, putative enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,786,768	0	C	T	100.0%	196.9 / NA	56	pseudogene (372/2253 nt)	ygaQ	uncharacterized protein, putative enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/36);  total (20/36)

ATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAT  >  NC_000913/2786634‑2786891                                                                                                                                       |                                                                                                                            aTTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCg                                                                                                                         <  2:254344/139‑1 (MQ=255)      ccTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGtt                                                                                                                    <  1:128538/139‑1 (MQ=255)        tttCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTa                                                                                                                  <  2:232079/139‑1 (MQ=255)         ttCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTAt                                                                                                                 <  2:791000/139‑1 (MQ=255)         ttCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTAt                                                                                                                 <  2:373817/139‑1 (MQ=255)                      aCTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTg                                                                                                    <  2:502688/139‑1 (MQ=255)                         tACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTg                                                                                                 <  1:250653/139‑1 (MQ=255)                           ccGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGaa                                                                                               >  2:153712/1‑139 (MQ=255)                                               cacaGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATc                                                                           >  1:598224/1‑139 (MQ=255)                                                   gCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGAc                                                                       <  2:315760/139‑1 (MQ=255)                                                         cATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCt                                                                 >  2:283336/1‑139 (MQ=255)                                                             aCAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTccc                                                             <  2:598224/139‑1 (MQ=255)                                                              cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTAt                                                                                                                 <  2:759058/86‑1 (MQ=255)                                                              cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTAt                                                                                                                 >  1:759058/1‑86 (MQ=255)                                                              cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTcccc                                                            >  1:689084/1‑139 (MQ=255)                                                              cAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTcccc                                                            >  1:107519/1‑139 (MQ=255)                                                               aGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCa                                                           <  1:153712/139‑1 (MQ=255)                                                                gcgcCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaa                                                          >  1:127459/1‑139 (MQ=255)                                                                 cgcCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaaa                                                         >  2:205479/1‑139 (MQ=255)                                                                    cAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCcaca                                                                                  <  1:497174/111‑1 (MQ=255)                                                                    cAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGAGTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCcaca                                                                                  >  2:497174/1‑111 (MQ=255)                                                                      gACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATa                                                    >  2:239790/1‑139 (MQ=255)                                                                            aTAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATg                                              <  2:220289/139‑1 (MQ=255)                                                                                  gaaagaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCat                                        <  1:509756/139‑1 (MQ=255)                                                                                  gaaagaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCat                                        <  1:260203/139‑1 (MQ=255)                                                                                   aaagaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCata                                       <  2:425772/139‑1 (MQ=255)                                                                                   aaagaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCata                                       <  2:127459/139‑1 (MQ=255)                                                                                   aaagaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCata                                       <  2:107519/139‑1 (MQ=255)                                                                                     agaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATg                                     >  2:724313/1‑139 (MQ=255)                                                                                     agaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATg                                     >  2:379149/1‑139 (MQ=255)                                                                                     agaaaAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATg                                     >  2:245347/1‑139 (MQ=255)                                                                                           aCATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTa                               <  1:283336/139‑1 (MQ=255)                                                                                               aGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAatat                                               >  2:532115/1‑119 (MQ=255)                                                                                               aGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAatat                                               <  1:532115/119‑1 (MQ=255)                                                                                                 gTTTATCAACTAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTc                         <  1:779301/139‑1 (MQ=255)                                                                                                   ttATCAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCt                       <  1:245347/139‑1 (MQ=255)                                                                                                       cAACGAGATACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACt                   <  1:707872/139‑1 (MQ=255)                                                                                                           gagaTACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCt                       >  1:107455/1‑131 (MQ=255)                                                                                                           gagaTACAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCt                       <  2:107455/131‑1 (MQ=255)                                                                                                                 cAATGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGc         <  2:297718/139‑1 (MQ=255)                                                                                                                   aTGATTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAg       <  1:239790/139‑1 (MQ=255)                                                                                                                      aTTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGGGCTGGCAGCCg    <  2:382077/139‑1 (MQ=255)                                                                                                                      aTTGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCg    <  2:428365/139‑1 (MQ=255)                                                                                                                       ttGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCcaca                                                                                  <  1:131047/60‑1 (MQ=255)                                                                                                                       ttGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCcaca                                                                                  >  2:131047/1‑60 (MQ=255)                                                                                                                       ttGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGa   >  1:799649/1‑139 (MQ=255)                                                                                                                        tGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAt  <  1:660089/139‑1 (MQ=255)                                                                                                                        tGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAt  <  1:724313/139‑1 (MQ=255)                                                                                                                        tGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAt  <  1:205479/139‑1 (MQ=255)                                                                                                                        tGAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAt  <  2:799649/139‑1 (MQ=255)                                                                                                                         gAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCg    >  1:391619/1‑136 (MQ=255)                                                                                                                         gAAAGAGGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCg    <  2:391619/136‑1 (MQ=255)                                                                                                                              agGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaa                                                          >  1:345374/1‑77 (MQ=255)                                                                                                                              agGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaa                                                          <  2:345374/77‑1 (MQ=255)                                                                                                                              agGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAt  <  1:805801/133‑1 (MQ=255)                                                                                                                              agGTGATAATAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAt  >  2:805801/1‑133 (MQ=255)                                                                                                                                       |                                                                                                                            ATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAACATTCCTGACTATGAGCTGGCAGCCGAT  >  NC_000913/2786634‑2786891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑