| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,159,844 | G→A | P435S (CCA→TCA) | ygiQ ← | Radical SAM superfamily protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,159,844 | 0 | G | A | 100.0% | 145.2 / NA | 43 | P435S (CCA→TCA) | ygiQ | Radical SAM superfamily protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/22); total (21/22) | |||||||||||
GATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGAAA > NC_000913/3159712‑3159974 | gATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACccacc < 2:80661/139‑1 (MQ=255) ggttcgtggttcgtGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGATCCACCAAGATCGGAAATCACGc > 2:116380/1‑139 (MQ=255) cgtggttcgtgTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCAAGGCGATTTGCAGCGCCACATATACCTGTTGTCAGTTGACGCACCAAGATCTGAAATCACGCCAGt > 2:260736/1‑139 (MQ=255) tcgtgTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAAcc < 1:47123/139‑1 (MQ=255) tgtCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTgg > 1:453709/1‑139 (MQ=255) tCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGaa < 1:403637/139‑1 (MQ=255) cGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTcg > 1:760266/1‑139 (MQ=255) gATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGATGACCCACCAAGATCAGAAATCACGCACGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGAt < 1:513462/139‑1 (MQ=255) aTAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAAcc > 2:23757/1‑111 (MQ=255) aTAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAAcc < 1:23757/111‑1 (MQ=255) aTAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGAtc > 2:27128/1‑139 (MQ=255) aaaCGCACGACAAACGCCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGAtctc > 2:141737/1‑139 (MQ=255) gACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGa > 2:754221/1‑139 (MQ=255) aCAAACGGCGAGAAGTTTGTGTAGCGCGGGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACACACCAAGATCGTAAATCACGCCCGTAAAACCTGGAACGGTGTGGCGGATCGCTTCGATCTCATTAATGAt < 2:708444/139‑1 (MQ=255) aaCGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGa < 1:27128/139‑1 (MQ=255) aCAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCg > 2:280062/1‑139 (MQ=255) aGTTTGTTCAGCGCGTGGCGATTTGCATCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGaa < 2:23183/139‑1 (MQ=255) aGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGaa < 1:324612/139‑1 (MQ=255) aGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGaa < 2:95266/139‑1 (MQ=255) tGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGc < 1:14391/139‑1 (MQ=255) cgcgTGGCGATTTGTAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGaat > 1:491882/1‑139 (MQ=255) cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTa > 2:645763/1‑110 (MQ=255) cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTa < 1:645763/110‑1 (MQ=255) cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGaat > 2:348429/1‑139 (MQ=255) cgcgTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGaat > 1:316491/1‑139 (MQ=255) gCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATgcg < 1:280062/139‑1 (MQ=255) gCGATTTGCAGCGCAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATgcg < 1:394662/139‑1 (MQ=255) gcAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGTTCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc < 1:754221/139‑1 (MQ=255) gcAACATATACATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc < 1:348429/139‑1 (MQ=255) aaCATATACATGTTGGCAGTTGATCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCgg < 1:116380/139‑1 (MQ=255) taCATGTTGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAg < 1:141737/139‑1 (MQ=255) ttGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGaa > 2:445072/1‑49 (MQ=255) ttGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGaa < 1:445072/49‑1 (MQ=255) ttGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGa > 1:727051/1‑139 (MQ=255) tGGCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGaa > 2:71753/1‑139 (MQ=255) ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTaaa > 1:748302/1‑39 (MQ=255) ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTaaa < 2:748302/39‑1 (MQ=255) ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACg > 2:670869/1‑49 (MQ=255) ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACg < 1:670869/49‑1 (MQ=255) ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGaaa > 1:367131/1‑139 (MQ=255) ggCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGaaa > 1:190081/1‑139 (MQ=255) gCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc > 1:709286/1‑122 (MQ=255) gCAGTTGACCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTc < 2:709286/122‑1 (MQ=255) | GATAGAGGTTGATCGTTGGTTCGTGGTTCGTGTCCATGTGCGGACAAATATCCGGATAAACGCACGACAAACGGCGACAAGTTTGTTCAGCGCGTGGCGATTTGCAGCGCAACATATACATGTTGGCAGTTGGCCCACCAAGATCGGAAATCACGCCCGTAAAACCTGGAACGGTGTCGCGGATCGCTTCGATCTCATTAATGATCGAATCTTCGGAACGGCTCTGAATAATGCGCCCTTCGTGCTCGGTGATAGAACAGAAA > NC_000913/3159712‑3159974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |