Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,827	C→T	A331T (GCC→ACC)	mtr ←	tryptophan transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,827	0	C	T	100.0%	72.5 / NA	22	A331T (GCC→ACC)	mtr	tryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

ATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACC  >  NC_000913/3304736‑3304960                                                                                            |                                                                                                                                      aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGa                                                                                        <  1:427912/139‑1 (MQ=255)  ttGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaa                                                                                       <  1:413694/139‑1 (MQ=255)   tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                                                                                      <  1:403038/139‑1 (MQ=255)       gccCAGATGGTAGCCGCTAAACAAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGACTCGTCGAAACCaa                                                                                  >  2:163228/1‑139 (MQ=255)                       cTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa                                                                  >  1:55025/1‑139 (MQ=255)                              aGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaac                                                           <  2:190703/139‑1 (MQ=255)                                cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTGAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaaacaa                                                         >  1:503667/1‑139 (MQ=255)                                   aaCCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAAcc                                                      <  2:55025/139‑1 (MQ=255)                                         tGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGt                                                >  2:23881/1‑139 (MQ=255)                                          ggCGTACAGGAATCCGTTCGGGAATAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATg                                                                                                                         >  2:546008/1‑65 (MQ=255)                                          ggCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATg                                                                                                                         <  1:546008/65‑1 (MQ=255)                                             gTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACg                                            >  2:230139/1‑139 (MQ=255)                                             gTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGCTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACg                                            >  2:785347/1‑139 (MQ=255)                                              tACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGc                                           >  1:778696/1‑139 (MQ=255)                                                      tCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCca                                                                                                       >  1:777050/1‑71 (MQ=255)                                                      tCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCca                                                                                                       <  2:777050/71‑1 (MQ=255)                                                      tCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaac                                   >  1:765549/1‑139 (MQ=255)                                                          ttCGGGAACAACAGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGgaacgaaa                               >  1:68173/1‑138 (MQ=255)                                                                  caacaGCCCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTAGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTAcc                       >  2:657946/1‑139 (MQ=255)                                                                        cccccccACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGcc                                                                                                         <  2:213099/51‑1 (MQ=255)                                                                        cccccccACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGcc                                                                                                         >  1:213099/1‑51 (MQ=255)                                                                                       cGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGAcc  <  1:23881/139‑1 (MQ=255)                                                                                            |                                                                                                                                      ATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAAACAAACCCAGCGTTACGCCGAGGAACGAACTCGCTACCGCAAAGTTTGAGAACACGACC  >  NC_000913/3304736‑3304960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑