| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 352,986 | A→G | N94S (AAC→AGC) | prpE → | propionate‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 352,986 | 0 | A | G | 100.0% | 88.4 / NA | 24 | N94S (AAC→AGC) | prpE | propionate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/11); total (13/11) | |||||||||||
GCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAT > NC_000913/352851‑353097 | gCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAgcgc > 2:207122/1‑139 (MQ=255) ccACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTg > 1:654870/1‑139 (MQ=255) ccATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCAtt > 1:248253/1‑139 (MQ=255) aCCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGgtgt > 2:277526/1‑139 (MQ=255) aGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGt < 2:654870/139‑1 (MQ=255) gCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTg < 1:339838/139‑1 (MQ=255) ggcgctgATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATg > 1:676418/1‑139 (MQ=255) gTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCg < 1:689802/139‑1 (MQ=255) ctctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGaa < 1:277526/139‑1 (MQ=255) ctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAgc < 2:432734/139‑1 (MQ=255) tCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCggg > 2:43353/1‑106 (MQ=255) tCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCggg < 1:43353/106‑1 (MQ=255) aCAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTg > 2:796183/1‑102 (MQ=255) aCAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTg < 1:796183/102‑1 (MQ=255) cgcACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTc > 2:183694/1‑62 (MQ=255) cgcACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTc < 1:183694/62‑1 (MQ=255) cACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTg > 1:575299/1‑55 (MQ=255) cACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTg < 2:575299/55‑1 (MQ=255) tacctttCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTgcgcgcgc > 1:506322/1‑139 (MQ=255) cctttCGTCAGCTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAt > 1:593244/1‑139 (MQ=255) cTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCg > 2:159620/1‑38 (MQ=255) cTGCATGACGAAGTGAGCGCGGTGGCCTCAATGTTGCg < 1:159620/38‑1 (MQ=255) aGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTg > 1:531479/1‑58 (MQ=255) aGTGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTg < 2:531479/58‑1 (MQ=255) tGAGCGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAt > 1:349895/1‑115 (MQ=255) | GCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCAT > NC_000913/352851‑353097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |