Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,748,181	G→A	A366A (GCG→GCA)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,181	0	G	A	100.0%	111.7 / NA	33	A366A (GCG→GCA)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/16);  total (17/16)

TCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGC  >  NC_000913/3748055‑3748314                                                                                                                               |                                                                                                                                      tcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAg                                                                                                                           <  2:294792/139‑1 (MQ=255)      ccGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAATGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCAt                                                                                                                      >  1:5967/1‑139 (MQ=255)                  tGCCGAAGGCTGGGGGCGATCTCTTTTCCCTGCCGGTCCTGTACGTCAGCCACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATATATGAAGGggt                                                                                                          <  2:342854/139‑1 (MQ=255)                   gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggtg                                                                                                         >  1:393302/1‑139 (MQ=255)                           cTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg                                                                                                 <  2:309645/139‑1 (MQ=255)                              ggggCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                                                                              <  2:393302/139‑1 (MQ=255)                                           tttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc                                                                                        <  2:705829/132‑1 (MQ=255)                                           tttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc                                                                                        >  1:705829/1‑132 (MQ=255)                                              ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg                                                                                                                          <  1:307480/95‑1 (MQ=255)                                              ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg                                                                                                                          >  2:307480/1‑95 (MQ=255)                                                 gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTc                                                                                                   <  1:32503/115‑1 (MQ=255)                                                 gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTc                                                                                                   >  2:32503/1‑115 (MQ=255)                                                 gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                                                                           >  1:40515/1‑139 (MQ=255)                                                     ttCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggtggt                                                                                                       >  1:696090/1‑107 (MQ=255)                                                     ttCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggtggt                                                                                                       <  2:696090/107‑1 (MQ=255)                                                       ccTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATgcgc                                                                     <  1:58758/139‑1 (MQ=255)                                                          gTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGaa                                                                  <  2:477911/139‑1 (MQ=255)                                                                cagcaACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGtttt                                                            >  2:37527/1‑139 (MQ=255)                                                                 agcaACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                                                                           >  1:312564/1‑123 (MQ=255)                                                                 agcaACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                                                                           <  2:312564/123‑1 (MQ=255)                                                                           gACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTca                                                 <  2:201340/139‑1 (MQ=255)                                                                                      aCCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                                                                                   <  2:284603/94‑1 (MQ=255)                                                                                      aCCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                                                                                   >  1:284603/1‑94 (MQ=255)                                                                                      aCCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTAcgcg                                      >  2:85372/1‑139 (MQ=255)                                                                                                tCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGcc                                                                                                                        >  2:102237/1‑47 (MQ=255)                                                                                                tCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGcc                                                                                                                        <  1:102237/47‑1 (MQ=255)                                                                                                       gATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAATGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGgcg                     <  2:570846/139‑1 (MQ=255)                                                                                                       gATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGgcg                     >  2:780596/1‑139 (MQ=255)                                                                                                         tGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCa                   >  2:319709/1‑139 (MQ=255)                                                                                                              gCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCg              <  1:319709/139‑1 (MQ=255)                                                                                                                 aTTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGTCCGGCGCACTCCGATg           >  1:153328/1‑139 (MQ=255)                                                                                                                   tCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTc         >  2:461593/1‑139 (MQ=255)                                                                                                                   tCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTc         >  2:662420/1‑139 (MQ=255)                                                                                                                          cgcgcaCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGc  <  1:461593/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                      TCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGC  >  NC_000913/3748055‑3748314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑