| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,131,253 | G→A | W473* (TGG→TGA) | metL → | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,131,253 | 0 | G | A | 100.0% | 99.5 / NA | 29 | W473* (TGG→TGA) | metL | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/13); total (16/13) | |||||||||||
GGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTT > NC_000913/4131119‑4131381 | ggCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTgg < 2:359957/139‑1 (MQ=255) gCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGtt < 1:581843/139‑1 (MQ=255) aGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTgag < 1:687277/139‑1 (MQ=255) gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTgagc > 1:277674/1‑139 (MQ=255) cgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGtt > 1:264147/1‑122 (MQ=255) cgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGtt < 2:264147/122‑1 (MQ=255) cGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCtt < 2:796781/139‑1 (MQ=255) gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc > 1:255666/1‑139 (MQ=255) aaaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAc > 1:105415/1‑139 (MQ=255) gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGaa > 1:79563/1‑65 (MQ=255) gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGaa < 2:79563/65‑1 (MQ=255) gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAg > 2:330955/1‑139 (MQ=255) gcgcAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAg > 2:240389/1‑139 (MQ=255) gAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgcc < 1:217744/139‑1 (MQ=255) cGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTg > 2:306155/1‑139 (MQ=255) ttGTTCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACg < 1:567038/139‑1 (MQ=255) ttCGGTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGc > 2:361821/1‑139 (MQ=255) gTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGa > 1:396865/1‑139 (MQ=255) gTAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGa < 1:330955/139‑1 (MQ=255) tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCt < 1:753052/87‑1 (MQ=255) tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCt > 2:753052/1‑87 (MQ=255) tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgc > 2:677412/1‑109 (MQ=255) tAAGGGCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgc < 1:677412/109‑1 (MQ=255) gggCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgcc > 1:507830/1‑107 (MQ=255) gggCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAgccgcc < 2:507830/107‑1 (MQ=255) ggCAATATCGGTTCCCGTTGACTGGAACTGTTCGcc < 2:234214/36‑1 (MQ=255) ggCAATATCGGTTCCCGTTGACTGGAACTGTTCGcc > 1:234214/1‑36 (MQ=255) gCAATATCGGTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAg > 2:418047/1‑139 (MQ=255) gTTCCCGTTGACTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGtt > 2:551455/1‑139 (MQ=255) | GGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTT > NC_000913/4131119‑4131381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |