Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,472	A→G	E498G (GAG→GGG)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,472	0	A	G	100.0%	63.2 / NA	18	E498G (GAG→GGG)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/7);  total (11/7)

CGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGA  >  NC_000913/4281355‑4281592                                                                                                                      |                                                                                                                         cggATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCg                                                                                                     >  2:513064/1‑139 (MQ=255)       ccGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTggc                                                                                               >  2:17537/1‑139 (MQ=255)           agagAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCa                                                                                           >  2:63881/1‑139 (MQ=255)            gagaATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAt                                                                                          <  1:510101/139‑1 (MQ=255)                    cGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCt                                                                                  >  2:668998/1‑139 (MQ=255)                         aCCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACg                                                                             >  2:19059/1‑139 (MQ=255)                                                  tcGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc                                                     >  2:777202/1‑138 (MQ=255)                                                  tcGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc                                                     <  1:777202/138‑1 (MQ=255)                                                   cgcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCca                                                   <  1:17537/139‑1 (MQ=255)                                                                     cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                 >  2:115457/1‑139 (MQ=255)                                                                     cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                 >  1:192634/1‑139 (MQ=255)                                                                                          aaCGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCa                                                                                           <  1:560049/60‑1 (MQ=255)                                                                                          aaCGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCa                                                                                           >  2:560049/1‑60 (MQ=255)                                                                                           acgacgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCa           <  1:762078/139‑1 (MQ=255)                                                                                           acgacgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCa           <  1:19059/139‑1 (MQ=255)                                                                                                  tGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc                                                     >  2:152363/1‑90 (MQ=255)                                                                                                  tGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc                                                     <  1:152363/90‑1 (MQ=255)                                                                                                    aaaGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGa  >  1:500513/1‑139 (MQ=255)                                                                                                                      |                                                                                                                         CGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGA  >  NC_000913/4281355‑4281592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑