Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,935	A→G	L69L (TTG→CTG)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,935	0	A	G	100.0%	168.1 / NA	45	L69L (TTG→CTG)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

TCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGAT  >  NC_000913/4490803‑4491064                                                                                                                                     |                                                                                                                                  tCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                             <  1:639225/139‑1 (MQ=255)   aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                           <  2:253223/139‑1 (MQ=255)   aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                           <  1:377207/139‑1 (MQ=255)                   aaTCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGc                                                                                                           >  2:638293/1‑139 (MQ=255)                     tCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCAt                                                                                                         >  2:663670/1‑139 (MQ=255)                        cgATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTca                                                                                                      >  1:367173/1‑139 (MQ=255)                                  tgtaattAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGcc                                                                                            <  2:270027/139‑1 (MQ=255)                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGta                                                                                                                      >  1:168073/1‑108 (MQ=255)                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGta                                                                                                                      <  2:168073/108‑1 (MQ=255)                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                       <  1:149185/139‑1 (MQ=255)                                          gCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTCTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAgcgc                                                                                    >  1:797915/1‑139 (MQ=255)                                              tAAGGGTCTGATAATTATGCTCAGAGGTGGCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAAACAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                                <  2:11467/139‑1 (MQ=255)                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                                <  2:797915/139‑1 (MQ=255)                                                aGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAg                                                                              <  2:684889/139‑1 (MQ=255)                                                   gTCTTATAAGTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                           <  2:44903/139‑1 (MQ=255)                                                     cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                             >  1:574160/1‑87 (MQ=255)                                                     cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                             <  2:574160/87‑1 (MQ=255)                                                            ttATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                           <  2:416536/82‑1 (MQ=255)                                                            ttATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                           >  1:416536/1‑82 (MQ=255)                                                            ttATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGa                                                                  >  1:196670/1‑139 (MQ=255)                                                            ttATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGa                                                                  >  1:295834/1‑139 (MQ=255)                                                               tGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAtct                                                               >  2:164651/1‑139 (MQ=255)                                                                gCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                           <  2:660387/126‑1 (MQ=255)                                                                gCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                           >  1:660387/1‑126 (MQ=255)                                                                    agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAgcgc                                                                                    <  2:308072/113‑1 (MQ=255)                                                                    agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAgcgc                                                                                    >  1:308072/1‑113 (MQ=255)                                                                    agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTcc                                                          >  1:740768/1‑139 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCg                                                                                           >  1:357197/1‑104 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCg                                                                                           <  2:357197/104‑1 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                        <  2:740768/139‑1 (MQ=255)                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                        <  1:638293/139‑1 (MQ=255)                                                                                ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCg                                              >  1:492594/1‑139 (MQ=255)                                                                                ttCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCg                                              >  2:628040/1‑139 (MQ=255)                                                                                          gcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTGTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                                >  1:479474/1‑95 (MQ=255)                                                                                          gcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTCTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                                <  2:479474/95‑1 (MQ=255)                                                                                           cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCAtt                                                                                                        >  1:741986/1‑70 (MQ=255)                                                                                           cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCAtt                                                                                                        <  2:741986/70‑1 (MQ=255)                                                                                             agaATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                           >  1:743306/1‑97 (MQ=255)                                                                                             agaATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                           <  2:743306/97‑1 (MQ=255)                                                                                                     aGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCttt                         >  1:267515/1‑139 (MQ=255)                                                                                                      gCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTg                        >  1:780845/1‑139 (MQ=255)                                                                                                        gAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGcc                      <  2:196670/139‑1 (MQ=255)                                                                                                         aaGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCa                     >  1:69538/1‑139 (MQ=255)                                                                                                                   tttGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCgg           <  2:492594/139‑1 (MQ=255)                                                                                                                            aCGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGat  <  1:663670/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  TCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGAT  >  NC_000913/4490803‑4491064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑