| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,493,661 | A→G | V249A (GTG→GCG) | idnD ← | L‑idonate 5‑dehydrogenase, NAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,493,661 | 0 | A | G | 100.0% | 60.2 / NA | 17 | V249A (GTG→GCG) | idnD | L‑idonate 5‑dehydrogenase, NAD‑binding |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/7); total (10/7) | |||||||||||
AGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACGAGCACATCCGCCCCCATCTCTTTGCCCAGCGAAAGGGAACG > NC_000913/4493542‑4493794 | agCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGa < 2:187040/139‑1 (MQ=255) atcatTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCCTTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCg < 1:667973/139‑1 (MQ=255) atcatTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCg < 2:36310/139‑1 (MQ=255) catTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCt > 1:555174/1‑139 (MQ=255) ggAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCa < 2:555174/139‑1 (MQ=255) cctGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTAc > 1:331825/1‑139 (MQ=255) cctGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTAc > 1:715478/1‑139 (MQ=255) ctGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACg > 1:482990/1‑139 (MQ=255) cACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACGAGc > 2:636652/1‑139 (MQ=255) tACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGAcc < 1:51726/54‑1 (MQ=255) tACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGAcc > 2:51726/1‑54 (MQ=255) cgcgTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACGAGCACATCCGc < 2:129101/136‑1 (MQ=255) cgcgTGCACGAGTGACCTCCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACGAGCACATCCGc > 1:129101/1‑136 (MQ=255) tGCACGAGTGACCACCAGACAGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACGAGCACATCCGCCCCCAtc > 2:650670/1‑139 (MQ=255) aGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCg > 2:411570/1‑63 (MQ=255) aGGTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCg < 1:411570/63‑1 (MQ=255) gTATTCGCTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACGAGCACATCCGCCCCCATCTC‑TTGCCCAGCGAAAGGGAACg > 2:633087/1‑139 (MQ=255) | AGCCTCTGAGTGAAATCTCCTTACCAATCAACGTCATCATTGGGAATTCTGCCATCGCGCCTCCCATACCTACCTGCACCATTACGCCGCGTGCACGAGTGACCTCCAGACAGGTATTCACTGATGAAGGATGACCGGACACTTCAAAGCTGACATCGAAATAGCCTTTTTCCGCTTTCCAGTGATCCATGTCGTCGTTTTGTGGGTTTACGAGCACATCCGCCCCCATCTCTTTGCCCAGCGAAAGGGAACG > NC_000913/4493542‑4493794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |