Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,553,049 A→G D50G (GAC→GGC)  uxuB → D‑mannonate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,553,0490AG100.0% 90.8 / NA 25D50G (GAC→GGC) uxuBD‑mannonate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/11);  total (14/11)

TTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGA  >  NC_000913/4552914‑4553174
                                                                                                                                       |                                                                                                                             
ttgACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAg                                                                                                                            >  1:769222/1‑139 (MQ=255)
       cAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTgg                                                                                                                     >  1:129037/1‑139 (MQ=255)
                          ccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAAcc                                                                                                  <  2:88395/139‑1 (MQ=255)
                          ccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAAcc                                                                                                  <  1:280698/139‑1 (MQ=255)
                                    aTCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAgg                                                                                        <  2:119048/139‑1 (MQ=255)
                                       aTTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCaa                                                                                     >  2:783270/1‑139 (MQ=255)
                                       aTTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCaa                                                                                     <  2:139823/139‑1 (MQ=255)
                                        ttCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAAc                                                                                    >  1:705056/1‑139 (MQ=255)
                                                               tgCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTAAACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCt                                                             >  1:68710/1‑139 (MQ=255)
                                                               tgCATCTCGGTTGCGGGGCATTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCt                                                             >  2:337970/1‑139 (MQ=255)
                                                                          tGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGc                                                  <  2:68710/139‑1 (MQ=255)
                                                                                                      cgcTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa                                                <  1:371078/113‑1 (MQ=255)
                                                                                                      cgcTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa                                                >  2:371078/1‑113 (MQ=255)
                                                                                                            ataCCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTg                                                                           <  2:254285/80‑1 (MQ=255)
                                                                                                            ataCCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTg                                                                           >  1:254285/1‑80 (MQ=255)
                                                                                                                 catcatCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCa           <  1:337970/139‑1 (MQ=255)
                                                                                                                   tcatcTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCAcc         >  2:260887/1‑139 (MQ=255)
                                                                                                                     atcTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa                                                >  1:726864/1‑98 (MQ=255)
                                                                                                                     atcTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa                                                <  2:726864/98‑1 (MQ=255)
                                                                                                                          ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGgc                     <  2:584645/120‑1 (MQ=255)
                                                                                                                          ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGgc                     >  1:584645/1‑120 (MQ=255)
                                                                                                                          ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGa  >  2:360662/1‑139 (MQ=255)
                                                                                                                          ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGa  >  1:18048/1‑139 (MQ=255)
                                                                                                                               aaGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa                                                <  2:181091/88‑1 (MQ=255)
                                                                                                                               aaGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa                                                >  1:181091/1‑88 (MQ=255)
                                                                                                                                       |                                                                                                                             
TTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGA  >  NC_000913/4552914‑4553174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑