Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 449,399 A→G F415L (TTC→CTC)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913449,3990AG100.0% 90.9 / NA 25F415L (TTC→CTC) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

GAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCC  >  NC_000913/449265‑449525
                                                                                                                                      |                                                                                                                              
gaaccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAgg                                                                                                                            <  1:652812/139‑1 (MQ=255)
      gaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTa                                                                                                                      >  2:426212/1‑139 (MQ=255)
                   ccccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGt                                                                                                         <  1:426212/139‑1 (MQ=255)
                                            cGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCAc                                                                                <  2:321105/139‑1 (MQ=255)
                                            cGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCAc                                                                                <  2:221607/139‑1 (MQ=255)
                                                         ccaccaGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCg                                                                   <  2:774907/139‑1 (MQ=255)
                                                             caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGt                                                                                              <  1:397508/108‑1 (MQ=255)
                                                             caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGt                                                                                              >  2:397508/1‑108 (MQ=255)
                                                             caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcag                                                                                      <  1:631258/116‑1 (MQ=255)
                                                             caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcag                                                                                      >  2:631258/1‑116 (MQ=255)
                                                             caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCa                                                               >  2:175639/1‑139 (MQ=255)
                                                                                    gcCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAg                                                                           <  1:439639/104‑1 (MQ=255)
                                                                                    gcCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAg                                                                           >  2:439639/1‑104 (MQ=255)
                                                                                     ccGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGa                                       >  2:456712/1‑139 (MQ=255)
                                                                                     ccGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGa                                       <  1:456712/139‑1 (MQ=255)
                                                                                               cgccgcCGATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAg                             >  2:742600/1‑139 (MQ=255)
                                                                                                  cgccgATGATCACGTTATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCAt                          >  2:151068/1‑139 (MQ=255)
                                                                                                                 tATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGaa                   <  1:574936/131‑1 (MQ=255)
                                                                                                                 tATGGAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGaa                   >  2:574936/1‑131 (MQ=255)
                                                                                                                    ggAAGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGAt        >  2:204275/1‑139 (MQ=255)
                                                                                                                      aaGTGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGc      <  1:371867/139‑1 (MQ=255)
                                                                                                                         tGCGCAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGc   >  2:16400/1‑139 (MQ=255)
                                                                                                                          gcgcAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc  <  1:151068/139‑1 (MQ=255)
                                                                                                                           cgcAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCca                                >  2:163085/1‑108 (MQ=255)
                                                                                                                           cgcAATCAGGAGCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCca                                <  1:163085/108‑1 (MQ=255)
                                                                                                                                      |                                                                                                                              
GAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCC  >  NC_000913/449265‑449525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: