Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,529	(A)6→7	intergenic (+169/+57)	ydgA → / ← uidC	DUF945 family protein/putative outer membrane porin for beta‑glucuronides porin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,523	1	.	A	100.0%	152.0 / NA	45	intergenic (+163/+63)	ydgA/uidC	DUF945 family protein/putative outer membrane porin for beta‑glucuronides porin protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (22/23);  total (22/23)

CACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑AAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTA  >  NC_000913/1691388‑1691655                                                                                                                                         |                                                                                                                                     cACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaa                                                                                                                                   <  1:710047/139‑1 (MQ=255)     ccGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaa                                                                                                                               >  1:495971/1‑138 (MQ=255)      cGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACGTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaac                                                                                                                              >  2:1050192/1‑137 (MQ=255)       ggCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaacg                                                                                                                             >  2:702741/1‑136 (MQ=255)                    gAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTcaa                                                                                                                 <  2:950650/139‑1 (MQ=255)                                   aTTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCg                                                                                                  >  1:938833/1‑139 (MQ=255)                                     tGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGga                                                                                                >  2:1114638/1‑139 (MQ=255)                                      gCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGgag                                                                                               >  1:802375/1‑139 (MQ=255)                                                cTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctct                                                                                     >  1:1195958/1‑139 (MQ=255)                                                 tACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCCCTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc                                                                                    <  1:123049/139‑1 (MQ=255)                                                 tACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc                                                                                    >  1:786372/1‑139 (MQ=255)                                                 tACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc                                                                                    <  2:647067/139‑1 (MQ=255)                                                      ttATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg                                                                               <  1:1050192/139‑1 (MQ=255)                                                        aTCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg                                                                               >  2:1113790/1‑137 (MQ=255)                                                        aTCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg                                                                               <  1:1113790/137‑1 (MQ=255)                                                         tCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTa                                                                            >  1:30776/1‑139 (MQ=255)                                                           aGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTAtt                                                                          >  1:190265/1‑139 (MQ=255)                                                             gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaacgcg                                                                                                                           >  2:662772/1‑82 (MQ=255)                                                             gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaaaacgcg                                                                                                                           <  1:662772/87‑6 (MQ=255)                                                             gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaa                                                                                                                                 >  2:73473/1‑81 (MQ=255)                                                             gCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑aaaaa                                                                                                                                 <  1:73473/81‑1 (MQ=255)                                                                  ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACt                                                                   <  1:209561/139‑1 (MQ=255)                                                                  ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACt                                                                   >  2:278990/1‑139 (MQ=255)                                                                   cTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACtt                                                                  <  2:1346252/139‑1 (MQ=255)                                                                        ccTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCa                                                             <  1:314565/139‑1 (MQ=255)                                                                               cacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTc                                                      <  1:702741/139‑1 (MQ=255)                                                                                  tttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa                                                   <  1:758605/139‑1 (MQ=255)                                                                                  tttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa                                                   <  2:1299322/139‑1 (MQ=255)                                                                                    tGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACt                                                 >  2:173456/1‑139 (MQ=255)                                                                                       aTTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGaa                                              <  1:795797/139‑1 (MQ=255)                                                                                           aTGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACt                                          <  2:30776/139‑1 (MQ=255)                                                                                            tGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTc                                         >  2:999910/1‑139 (MQ=255)                                                                                                   ttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTca                                  <  1:173456/139‑1 (MQ=255)                                                                                                      tAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACAt                               <  2:802375/139‑1 (MQ=255)                                                                                                           tGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAg                          >  2:720785/1‑139 (MQ=255)                                                                                                             gATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTca                                  >  1:817630/1‑129 (MQ=255)                                                                                                             gATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTca                                  <  2:817630/129‑1 (MQ=255)                                                                                                             gATAAGGCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTa                        >  1:1103632/1‑139 (MQ=255)                                                                                                                  ggCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg                                                                               >  2:1416410/1‑79 (MQ=255)                                                                                                                  ggCGTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCg                                                                               <  1:1416410/79‑1 (MQ=255)                                                                                                                     gTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc                                                                                    >  2:644934/1‑71 (MQ=255)                                                                                                                     gTTTACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctctc                                                                                    <  1:644934/71‑1 (MQ=255)                                                                                                                       ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCgg                                                                                                 <  2:247943/56‑1 (MQ=18)                                                                                                                       ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCgg                                                                                                 >  1:247943/1‑56 (MQ=18)                                                                                                                       ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCg                                                                                                  <  2:340085/55‑1 (MQ=18)                                                                                                                       ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCg                                                                                                  >  1:340085/1‑55 (MQ=18)                                                                                                                       ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCg               >  2:354387/1‑138 (MQ=255)                                                                                                                       ttACGCCGCATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCg               <  1:354387/138‑1 (MQ=255)                                                                                                                            ccgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa                                                   <  2:593450/97‑1 (MQ=255)                                                                                                                            ccgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATa                                                   >  1:593450/1‑97 (MQ=255)                                                                                                                             cgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGccc        >  2:1075904/1‑139 (MQ=255)                                                                                                                             cgcATCCGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGccc        >  1:77071/1‑139 (MQ=255)                                                                                                                                   cGGCATAAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTa  <  2:190265/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAT‑AAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTA  >  NC_000913/1691388‑1691655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑