Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,777	+G	coding (1718/3099 nt)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,774	1	.	G	100.0%	181.6 / NA	49	A572G (GCG→GGG)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (23/26);  total (23/26)

AAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGC‑GGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGA  >  NC_000913/3017651‑3017905                                                                                                                             |                                                                                                                                    aaaaaGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTa                                                                                                                       <  1:517136/139‑1 (MQ=255) aaaaaGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTa                                                                                                                       <  1:345042/139‑1 (MQ=255)          gggATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTg                                                                                                              >  1:1210131/1‑139 (MQ=255)              tGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAAcg                                                                                                          <  1:1208488/139‑1 (MQ=255)                          aCGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgc                                                                                              >  2:827077/1‑139 (MQ=255)                          aCGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgc                                                                                              >  2:592275/1‑139 (MQ=255)                          aCGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgc                                                                                              >  2:719771/1‑139 (MQ=255)                          aCGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGC‑gggg                                                                                                                                 >  1:1061146/1‑102 (MQ=255)                          aCGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGC‑gggg                                                                                                                                 <  2:1061146/103‑2 (MQ=255)                           cGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACg                                                                                                                     <  2:801112/115‑1 (MQ=255)                           cGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACg                                                                                                                     >  1:801112/1‑115 (MQ=255)                           cGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgcg                                                                                             >  2:435328/1‑139 (MQ=255)                           cGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgcg                                                                                             >  1:823264/1‑139 (MQ=255)                            gCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgcgg                                                                                            >  1:277677/1‑139 (MQ=255)                                      aCCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTg                                                                                                              >  2:134852/1‑111 (MQ=255)                                      aCCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTg                                                                                                              <  1:134852/111‑1 (MQ=255)                                       ccAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCgtggtg                                                                                 <  2:823264/139‑1 (MQ=255)                                       ccAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCgtggtg                                                                                 <  1:296812/139‑1 (MQ=255)                                         aGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGaa                                                                               <  2:1139789/139‑1 (MQ=255)                                                      tCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCt                                                                  <  2:1204732/139‑1 (MQ=255)                                                              tCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCg                                                          <  1:199465/139‑1 (MQ=255)                                                              tCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCg                                                          >  1:302878/1‑139 (MQ=255)                                                              tCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCg                                                          <  1:1260304/139‑1 (MQ=255)                                                                cGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTa                                                        <  2:408376/139‑1 (MQ=255)                                                                       cGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCGTCAGTTCCGTATTCCTGc                                                 <  1:1219757/139‑1 (MQ=255)                                                                               gTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTaa                                         >  1:36269/1‑139 (MQ=255)                                                                                tGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgcggg                                                                                           <  1:1356916/88‑1 (MQ=255)                                                                                tGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATgcggg                                                                                           >  2:1356916/1‑88 (MQ=255)                                                                                tGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTc                                                            <  1:1011833/119‑1 (MQ=255)                                                                                tGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTc                                                            >  2:1011833/1‑119 (MQ=255)                                                                                     gTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATc                                                                                                                             >  2:558527/1‑49 (MQ=38)                                                                                     gTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATc                                                                                                                             <  1:558527/49‑1 (MQ=38)                                                                                     gTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAGCGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGc                                   >  2:1001477/1‑139 (MQ=255)                                                                                     gTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCgt                                                                                     >  2:166840/1‑89 (MQ=255)                                                                                     gTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCgt                                                                                     <  1:166840/89‑1 (MQ=255)                                                                                      tCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCa                                  <  1:830097/139‑1 (MQ=255)                                                                                      tCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCa                                  <  2:277677/139‑1 (MQ=255)                                                                                        cggcggGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACg                                >  2:670359/1‑139 (MQ=255)                                                                                         ggcggGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGaaa                                                                              <  2:153635/92‑1 (MQ=255)                                                                                         ggcggGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGaaa                                                                              >  1:153635/1‑92 (MQ=255)                                                                                         ggcggGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGa                               <  2:36269/139‑1 (MQ=255)                                                                                          gcggGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAATCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGc                                                 <  1:990854/120‑1 (MQ=255)                                                                                          gcggGTCTGGCAGCAGGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGc                                                 >  2:990854/1‑120 (MQ=255)                                                                                                        agGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGAtt                       >  1:700072/1‑132 (MQ=255)                                                                                                        agGTTACTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGAtt                       <  2:700072/132‑1 (MQ=255)                                                                                                              cTTCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCa          <  1:121628/139‑1 (MQ=255)                                                                                                               ttCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCAc         >  2:522852/1‑139 (MQ=255)                                                                                                               ttCCTTGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCAc         <  1:719771/139‑1 (MQ=255)                                                                                                                    tGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCTGGCGGCGTGGTGAAAAATATCATTCCt                                                                  >  1:368992/1‑77 (MQ=255)                                                                                                                    tGCCAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCt                                                                  <  2:368992/77‑1 (MQ=255)                                                                                                                      ccAGAGCGGGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGa  >  1:617611/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                    AAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGCGGGTCTGGCAGCAGGTTACTTCCTTGCCAGAGC‑GGGCCATCCGGTTACGCTGTTTGAACGCGAAGCCAATGCGGGCGGCGTGGTGAAAAATATCATTCCTCAGTTCCGTATTCCTGCAGAGTTAATTCAGCACGATATCGATTTTGTTGCCGCTCACGGCGTGA  >  NC_000913/3017651‑3017905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑