Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,280,409	(A)7→8	coding (430/1650 nt)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,280,402	1	.	A	100.0%	183.8 / NA	52	P141P (CCG→CCA)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (24/28);  total (24/28)

ACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGT  >  NC_000913/4280265‑4280527                                                                                                                                           |                                                                                                                              aCCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑a                                                                                                                              >  1:1020616/1‑139 (MQ=255)  ccGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑aa                                                                                                                             <  2:1194524/139‑1 (MQ=255)  ccGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑aa                                                                                                                             >  1:161195/1‑139 (MQ=255)     tGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑aaaaa                                                                                                                          >  2:1280150/1‑139 (MQ=255)               tGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATggg                                                                                                                 >  1:1029568/1‑139 (MQ=255)               tGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATggg                                                                                                                 >  1:1199058/1‑139 (MQ=255)               tGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATggg                                                                                                                 >  1:373811/1‑139 (MQ=255)                             aCTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCg                                                                                                   <  2:120322/139‑1 (MQ=255)                               tGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGaa                                                                                                 <  2:976779/139‑1 (MQ=255)                               tGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGaa                                                                                                 >  2:510037/1‑139 (MQ=255)                               tGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGaa                                                                                                 <  2:1029568/139‑1 (MQ=255)                               tGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGaa                                                                                                 <  2:1020616/139‑1 (MQ=255)                                gggTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAg                                                                                                >  1:739262/1‑139 (MQ=255)                                gggTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAg                                                                                                >  1:193169/1‑139 (MQ=255)                                 ggtggtGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCg                                                                                                   <  2:166071/135‑1 (MQ=255)                                 ggtggtGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGc                                                                                               >  1:1006544/1‑139 (MQ=255)                                 ggtggtGCCGGGCATTCCGCTGATCCCTGCCTTTACGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCg                                                                                                   >  1:166071/1‑135 (MQ=255)                                     gtgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTtg                                                                                           <  2:1006544/139‑1 (MQ=255)                                      tgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTtga                                                                                          >  2:417332/1‑139 (MQ=255)                                      tgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTG‑TTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTtgat                                                                                         >  1:1317646/1‑139 (MQ=255)                                           ggCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGatgaat                                                                                     <  1:510037/139‑1 (MQ=255)                                           ggCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGatgaat                                                                                     >  2:1452165/1‑139 (MQ=255)                                             cATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGatgaatga                                                                                   <  1:657845/139‑1 (MQ=255)                                             cATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGatgaatga                                                                                   <  1:670957/139‑1 (MQ=255)                                                tCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGc                                                                                <  1:417332/139‑1 (MQ=255)                                                   gCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTTCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTc                                                                             <  2:1395321/139‑1 (MQ=255)                                                     tGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑aaaaaaaatcat                                                                                                                   <  1:861231/98‑6 (MQ=255)                                                     tGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑aaaaaaaatcat                                                                                                                   >  2:861231/1‑93 (MQ=255)                                                           cTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTgg                                                                     <  2:193169/139‑1 (MQ=255)                                                           cTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTgg                                                                     <  1:1452165/139‑1 (MQ=255)                                                            tGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGgt                                                                    >  1:104656/1‑139 (MQ=255)                                                             gCCTTTGCGCTGGCGGCGGTG‑TTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGgtgt                                                                  <  2:1317646/139‑1 (MQ=255)                                                                   gcgcTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTg                                                             >  1:562472/1‑139 (MQ=255)                                                                             cggTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCg                                                   <  2:161195/139‑1 (MQ=255)                                                                                          gaccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGAt                                      <  2:373811/139‑1 (MQ=255)                                                                                          gaccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGAt                                      <  2:12809/139‑1 (MQ=255)                                                                                               aTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCa                                                                                                               >  1:1204587/1‑61 (MQ=255)                                                                                               aTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCa                                                                                                               <  2:1204587/61‑1 (MQ=255)                                                                                               aTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTc                                                                        >  1:1220125/1‑100 (MQ=255)                                                                                               aTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTc                                                                        <  2:1220125/100‑1 (MQ=255)                                                                                                 gCTGTGGCGCTCTCCGGGATTGTTGGCGAGGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGa                                       <  1:1191498/131‑1 (MQ=255)                                                                                                 gCTGTGGCGCTCTCCGGGATTGTTGGCGAGGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGa                                       >  2:1191498/1‑131 (MQ=255)                                                                                                     tgGCGCTCTGCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACgatga                           >  1:984086/1‑139 (MQ=255)                                                                                                          ctctCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCt                                                                              <  1:119470/83‑1 (MQ=255)                                                                                                          ctctCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCt                                                                              >  2:119470/1‑83 (MQ=255)                                                                                                               cGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGt                 >  2:1088821/1‑139 (MQ=255)                                                                                                               cGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGt                 <  2:104656/139‑1 (MQ=255)                                                                                                                  gATTGTTGGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTcgg              <  1:1280150/139‑1 (MQ=255)                                                                                                                       ttgGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTtgatg                                                                                        <  1:1457462/60‑1 (MQ=255)                                                                                                                       ttgGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTtgatg                                                                                        >  2:1457462/1‑60 (MQ=255)                                                                                                                       ttgGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGAt                                      >  2:125882/1‑110 (MQ=255)                                                                                                                       ttgGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGAt                                      <  1:125882/110‑1 (MQ=255)                                                                                                                       ttgGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACgatgat                          >  2:1357515/1‑122 (MQ=255)                                                                                                                       ttgGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACgatgat                          <  1:1357515/122‑1 (MQ=255)                                                                                                                       ttgGCGAAGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTg         >  2:1075923/1‑139 (MQ=255)                                                                                                                              aGGGCGCATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGt  <  1:886617/139‑1 (MQ=255)                                                                                                                                 gcgcATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCt                                                                              >  1:886424/1‑60 (MQ=255)                                                                                                                                 gcgcATCCCGAAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCt                                                                              <  2:886424/60‑1 (MQ=255)                                                                                                                                           |                                                                                                                              ACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCG‑AAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGT  >  NC_000913/4280265‑4280527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑