Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	87,468	A→G	T38A (ACC→GCC)	ilvH →	acetolactate synthase 3, small subunit, valine‑sensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	87,468	0	A	G	100.0%	254.6 / NA	68	T38A (ACC→GCC)	ilvH	acetolactate synthase 3, small subunit, valine‑sensitive
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/37);  total (31/37)

AAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGC  >  NC_000913/87333‑87593                                                                                                                                        |                                                                                                                              aaGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCcg                                                                                                                            >  1:1463126/1‑139 (MQ=255)     aaaaCGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCcgacga                                                                                                                        <  1:12930/139‑1 (MQ=255)         cGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCg                                                                                                                    >  2:651170/1‑139 (MQ=255)               gaACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTa                                                                                                              <  2:320688/139‑1 (MQ=255)               gaACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTa                                                                                                              <  2:212600/139‑1 (MQ=255)               gaACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTa                                                                                                              <  2:942956/139‑1 (MQ=255)               gaACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTa                                                                                                              <  1:651170/139‑1 (MQ=255)                aaCCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTAt                                                                                                             <  2:1463126/139‑1 (MQ=255)                    tGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATcgcg                                                                                                         >  1:1252574/1‑139 (MQ=255)                        tATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     <  2:110119/139‑1 (MQ=255)                        tATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     <  1:170822/139‑1 (MQ=255)                         aTGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGa                                                                                                    <  2:922423/139‑1 (MQ=255)                         aTGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGa                                                                                                    <  1:41603/139‑1 (MQ=255)                             gcCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGa                                                                                                    <  1:1435360/135‑1 (MQ=255)                             gcCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGa                                                                                                    >  2:1435360/1‑135 (MQ=255)                                ggATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCa                                                                                             >  1:1159871/1‑139 (MQ=255)                                         cAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTggg                                                                                    <  2:1159871/139‑1 (MQ=255)                                         cAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTggg                                                                                    >  2:401209/1‑139 (MQ=255)                                           gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTAt                                                                                                             <  1:1124550/112‑1 (MQ=255)                                           gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTAt                                                                                                             >  2:1124550/1‑112 (MQ=255)                                           gTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCg                                                                                  >  2:1331794/1‑139 (MQ=255)                                           gTCTTACTCGAAAATGAATCAGGCGCGTTATACCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCTGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCg                                                                                  >  1:1086957/1‑139 (MQ=255)                                            tCTTACTCGTAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAGCATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     <  1:993356/119‑1 (MQ=255)                                            tCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     >  2:993356/1‑119 (MQ=255)                                            tCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGa                                                                                 >  2:567202/1‑139 (MQ=255)                                             cTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATcgcg                                                                                                         >  2:512035/1‑114 (MQ=255)                                             cTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATcgcg                                                                                                         <  1:512035/114‑1 (MQ=255)                                             cTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGAt                                                                                <  2:918715/139‑1 (MQ=255)                                                aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCcgacga                                                                                                                        <  2:84203/96‑1 (MQ=255)                                                aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCcgacga                                                                                                                        >  1:84203/1‑96 (MQ=255)                                                aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATc                                                                                               >  2:738989/1‑121 (MQ=255)                                                aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATc                                                                                               <  1:738989/121‑1 (MQ=255)                                                aCTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGaa                                                                             >  2:214279/1‑139 (MQ=255)                                                 cTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTa                                                                                                              <  1:646217/105‑1 (MQ=255)                                                 cTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTa                                                                                                              >  2:646217/1‑105 (MQ=255)                                                  tCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaa                                                                           <  1:1224327/139‑1 (MQ=255)                                                  tCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaa                                                                           <  2:1252574/139‑1 (MQ=255)                                                  tCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaa                                                                           <  1:1331794/139‑1 (MQ=255)                                                     aaaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGt                                                                        >  2:979517/1‑139 (MQ=255)                                                      aaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGAc                                                                                                   <  1:324178/111‑1 (MQ=255)                                                      aaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGAc                                                                                                   >  2:324178/1‑111 (MQ=255)                                                      aaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTa                                                                       >  2:1122320/1‑139 (MQ=255)                                                      aaaTGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTa                                                                       >  1:828816/1‑139 (MQ=255)                                                           aaTCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGa                                                                  >  2:290374/1‑139 (MQ=255)                                                              cAGGCGCGTTAACCCGCGTGATTGGCCTTTTTACCCAGCGGGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCa                                                               <  2:100719/139‑1 (MQ=255)                                                                    cgTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGa                                                         >  2:494267/1‑139 (MQ=255)                                                                          cccGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa                                                   <  1:1122320/139‑1 (MQ=255)                                                                              cgTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     >  2:580944/1‑85 (MQ=255)                                                                              cgTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     <  1:580944/85‑1 (MQ=255)                                                                              cgTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTa                                               <  1:214279/139‑1 (MQ=255)                                                                                            tctCCGAGGGTTGCTACAACATTGAAAGCCGCACCGTTGCGCCAGCCGTCGATCCGACATTATCGCGGATGACCAGCCAGACCGTGGGCGATGAAAAAGTACTTGGGCAGATCGAAAACCAATTACACAAACAGGTCGa                                 <  2:862770/137‑1 (MQ=255)                                                                                                ccAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATc                                                                                                                      <  1:835314/50‑1 (MQ=255)                                                                                                ccAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATc                                                                                                                      >  2:835314/1‑50 (MQ=255)                                                                                                   gCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTg                          >  1:434937/1‑139 (MQ=255)                                                                                                     gTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTgcg                        <  1:494267/139‑1 (MQ=255)                                                                                                            caacaTTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCgtgagt                 <  1:401209/139‑1 (MQ=255)                                                                                                              acaTTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     >  2:476252/1‑53 (MQ=255)                                                                                                              acaTTGAAAGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATg                                                                                                     <  1:476252/53‑1 (MQ=255)                                                                                                                      aGCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGc       <  1:1231003/139‑1 (MQ=255)                                                                                                                       gCCTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCa      <  2:658437/139‑1 (MQ=255)                                                                                                                         cTGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAgg    >  2:587853/1‑139 (MQ=255)                                                                                                                          tGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa                                                   >  2:936229/1‑91 (MQ=255)                                                                                                                          tGACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCa                                                   <  1:936229/91‑1 (MQ=255)                                                                                                                           gACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaa                                                                           >  2:1101988/1‑66 (MQ=255)                                                                                                                           gACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGaaaa                                                                           <  1:1101988/66‑1 (MQ=255)                                                                                                                           gACCGTTGCGCCAGCCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGgc  >  2:1085665/1‑139 (MQ=255)                                                                                                                            aCCGTTGCGCCAGCCGACGATCCGACATTATcgcg                                                                                                         >  2:1130432/1‑35 (MQ=255)                                                                                                                            aCCGTTGCGCCAGCCGACGATCCGACATTATcgcg                                                                                                         <  1:1130432/35‑1 (MQ=255)                                                                                                                                        |                                                                                                                              AAGCAAAACGGAGAGAACCTGATTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGATCCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATCGAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGC  >  NC_000913/87333‑87593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑