Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,089,446 C→G A138P (GCC→CCC)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,089,4460CG100.0% 204.0 / NA 53A138P (GCC→CCC) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (23/30);  total (23/30)

AAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTC  >  NC_000913/1089311‑1089559
                                                                                                                                       |                                                                                                                 
aaGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCa                                                                                                                <  2:707803/139‑1 (MQ=255)
  gCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATa                                                                                                              >  1:1409546/1‑139 (MQ=255)
      gCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATAcagc                                                                                                          <  1:1390693/139‑1 (MQ=255)
      gCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATAcagc                                                                                                          <  1:336153/139‑1 (MQ=255)
       cTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATAcagca                                                                                                         <  1:319575/139‑1 (MQ=255)
         cAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAgg                                                                                                       <  2:151544/139‑1 (MQ=255)
               gCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTg                                                                                                 >  2:498903/1‑139 (MQ=255)
                ccGGGAACGAGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTgg                                                                                                <  1:939052/139‑1 (MQ=255)
                        gcgcAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTg                                                                                        <  2:240883/139‑1 (MQ=255)
                         cgcAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTga                                                                                       >  1:487816/1‑139 (MQ=255)
                                          gttgCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcgcg                                                                      <  2:1150215/139‑1 (MQ=255)
                                          gttgCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcgcg                                                                      <  2:1004001/139‑1 (MQ=255)
                                          gttgCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcgcg                                                                      <  1:12983/139‑1 (MQ=255)
                                           ttgCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCgg                                                                     <  2:1155861/139‑1 (MQ=255)
                                            tgCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCggg                                                                    <  2:1102582/139‑1 (MQ=255)
                                                   tGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTgaagaa                                                                                   <  2:736848/117‑1 (MQ=255)
                                                   tGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTgaagaa                                                                                   >  1:736848/1‑117 (MQ=255)
                                                                tcgatcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTgaa                                                                                      >  1:731152/1‑101 (MQ=255)
                                                                tcgatcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTgaa                                                                                      <  2:731152/101‑1 (MQ=255)
                                                                  gatcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAc                                                                         <  1:62684/112‑1 (MQ=255)
                                                                  gatcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAc                                                                         >  2:62684/1‑112 (MQ=255)
                                                                   atcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGtat                                                                 <  1:418148/119‑1 (MQ=255)
                                                                   atcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGtat                                                                 >  2:418148/1‑119 (MQ=255)
                                                                      gaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGtcatca                                          <  2:1409546/139‑1 (MQ=255)
                                                                       aCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATAcag                                                                                                           <  2:199014/73‑1 (MQ=255)
                                                                       aCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATAcag                                                                                                           >  1:199014/1‑73 (MQ=255)
                                                                          aaCTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAg                                      >  1:307203/1‑139 (MQ=255)
                                                                                tCGCCAAATTTTACTTGTTTATCCGCTGGTGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcgcg                                                                      <  1:1325311/101‑1 (MQ=255)
                                                                                tCGCCAAATTTTACTTGTTTATCCGCTGGTGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcgcg                                                                      >  2:1325311/1‑101 (MQ=255)
                                                                                 tgCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCAc                               >  1:819429/2‑139 (MQ=255)
                                                                                 cGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCAc                               >  1:487074/1‑139 (MQ=255)
                                                                                 cGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCAc                               <  2:307203/139‑1 (MQ=255)
                                                                                  gCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTgg                                                                                                <  2:966385/73‑1 (MQ=255)
                                                                                  gCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTgg                                                                                                >  1:966385/1‑73 (MQ=255)
                                                                                      aaTTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAg                          >  2:1330154/1‑139 (MQ=255)
                                                                                           tACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTg                                                                                        >  2:454051/1‑72 (MQ=255)
                                                                                           tACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTg                                                                                        <  1:454051/72‑1 (MQ=255)
                                                                                             cTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTc                   <  1:498903/139‑1 (MQ=255)
                                                                                                  ttATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTa              >  2:898633/1‑139 (MQ=255)
                                                                                                   tATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTgg                                                                                                >  1:1202664/1‑56 (MQ=255)
                                                                                                   tATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTgg                                                                                                <  2:1202664/56‑1 (MQ=255)
                                                                                                   tATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGcc                                                 <  2:418391/103‑1 (MQ=255)
                                                                                                   tATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGcc                                                 >  1:418391/1‑103 (MQ=255)
                                                                                                   tATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAg             <  1:1330154/139‑1 (MQ=255)
                                                                                                    aTCCGCTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGc            <  1:898633/139‑1 (MQ=255)
                                                                                                         cTGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGttt       >  2:1258268/1‑139 (MQ=255)
                                                                                                          tGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGtttt      >  2:1001244/1‑139 (MQ=255)
                                                                                                          tGGCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGtttt      >  2:925602/1‑139 (MQ=255)
                                                                                                            gCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGa                                                                                               >  2:155890/1‑48 (MQ=255)
                                                                                                            gCGTATCGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGa                                                                                               <  1:155890/48‑1 (MQ=255)
                                                                                                              gTAACGACCCAACTGCCGACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTtcctc  <  1:1066182/139‑1 (MQ=255)
                                                                                                                               gACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGt                                     >  2:693454/1‑87 (MQ=255)
                                                                                                                               gACGGGGGGCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGt                                     <  1:693454/87‑1 (MQ=255)
                                                                                                                                       |                                                                                                                 
AAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTC  >  NC_000913/1089311‑1089559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑